[12] combined sNuc-Seq with microfluidic technology to introduce a single-cell nuclear RNA sequencing method with low cost, high-sensitive and high-efficiency cell classification, which is expected to be used in human cell mapping projects (Table 1). Lake et al. [13] combined single-core ...
Single cardiomyocyte nuclear transcriptomes reveal a lincRNA-regulated de-differentiation and cell cycle stress-response in vivo. Nat. Commun. 8, 225 (2017). Article PubMed PubMed Central CAS Google Scholar Gao, R. et al. Nanogrid single-nucleus RNA sequencing reveals phenotypic diversity in ...
The brief workflow of single-cell RNA sequencing for these orthopedic disorders Full size image ScRNA-seq has four major advantages over bulk RNA-seq in biological research. First, this approach can reveal cellular heterogeneity or specific subclusters; second, it can depict the trajectories of diff...
Nuclear transcriptomes at high resolution using retooled INTACT Plant Physiol., 176 (2018), pp. 270-281 CrossrefView in ScopusGoogle Scholar Ryu et al., 2019 K.H. Ryu, L. Huang, H.M. Kang, J. Schiefelbein Single-cell RNA sequencing resolves molecular relationships among individual plant cel...
Here, we used single-cell RNA sequencing (scRNA-seq) to decipher the composition and functions of GAMs in murine experimental GL261 gliomas grown in male and female mice. We demonstrate distinct transcriptional programs of microglia (MG), monocytes/macrophages (Mo/MΦ), and CNS BAMs. The ident...
We present a snRNASeq protocol and compare its results with scRNA-seq. Two nuclear suspensions were prepared in lysis buffer on ice while one cell suspension was generated using enzymatic and mechanical dissociation. Cells and nuclei were processed using the 10x Genomics platform, and sequencing ...
multiplexing using lipid-tagged indices for single-cell and single-nucleus RNA sequencing. MULTI-seq reagents can barcode any cell type or nucleus from any species with an accessible plasma membrane. The method involves minimal sample processing, thereby preserving cell viability and endogenous gene exp...
the lower quantity of RNA per cell and smaller number of sampled cells in the single nuclear data provided only a higher-order perspective in the published analysis. Our higher-resolution model enables us to more precisely map the microglial subset implicated in AD and to guide the selection of...
Prostate cancer is heterogeneous and patients would benefit from methods that stratify those who are likely to respond to systemic therapy. Here, we employ single-cell assays for transposase-accessible chromatin (ATAC) and RNA sequencing in models of ear
J. in Single Cell Methods: Sequencing and Proteomics (ed. Proserpio, V.) 9–21 (Springer, 2019). See, K. et al. Single cardiomyocyte nuclear transcriptomes reveal a lincRNA-regulated de-differentiation and cell cycle stress-response in vivo. Nat. Commun. 8, 225 (2017). PubMed PubMed ...