#科研# 单细胞核RNA测序(single-nucleus RNA sequencing, snRNA-seq);10x Genomics和BD Rhapsody单细胞测序平台O一篇文章终结你对单细胞核测序的所有困惑 一篇文章终结你对单细胞核测序的所有困惑 单细胞测序(single-cell sequencing)是解析生物学现象最强有力的技术手段 自19世纪30年代......
Cite: Habib N, Li Y, Heidenreich M. et al. Div-Seq: Single-nucleus RNA-Seq reveals dynamics of rare adult newborn neurons.Science. 2016 Aug 26;353(6302):925-8. Abstract 单细胞 RNA 测序提供了有关细胞类型和状态的丰富信息。然而,捕获成体神经元发生等罕见动态过程非常困难,因为从成体组织中分...
Functional diversities within neurons and astrocytes in the adult rat auditory cortex revealed by single-nucleus RNA sequencing The mammalian cerebral cortex is composed of a rich diversity of cell types. Sensory cortical cells are organized into networks that rely on their function... A Gungor Aydin...
Single-cell RNA sequencing (RNA-Seq) provides rich information about cell types and states. However, it is difficult to capture rare dynamic processes, such as adult neurogenesis, because isolation of rare neurons from adult tissue is challenging and markers for each phase are limited. Here, we...
Defining cellular and molecular identities within the kidney is necessary to understand its organization and function in health and disease. Here we demonstrate a reproducible method with minimal artifacts for single-nucleus Droplet-based RNA sequencing
First, we used single-nucleus RNA sequencing to estimate RNA velocity. Compared to single-cell sequencing, snRNA-seq is more amenable to transcriptomic profiling of postmortem samples because isolated nuclei are intact in frozen tissues29. Moreover, dissociating whole cells from the brain is ...
As a necessary step in gaining a deeper understanding of the regulatory mechanisms controlling plant gene expression, we report the use of single-nucleus RNA sequencing (sNucRNA-seq) and single-nucleus assay for transposase accessible chromatin sequencing (sNucATAC-seq) technologies on Arabidopsis ...
Single-nucleus RNA sequencing of human pancreatic islets identifies novel gene sets and distinguishes β-cell subpopulations with dynamic transcriptome profiles. Genome Med. 2023;15(1):30. Article CAS PubMed PubMed Central Google Scholar Martelotto LG, Martelotto L. “Frankenstein” protocol for ...
有一部分细胞核也编码线粒体相关基因。虽然现存生物体中绝大多数作用于线粒体的蛋白质，是由细胞核DNA...
Single-nucleus RNA sequencing (sNuc-seq) profiles RNA from tissues that are preserved or cannot be dissociated, but it does not provide high throughput. Here, we develop DroNc-seq: massively parallel sNuc-seq with droplet technology. We profile 39,111 nuclei from mouse and human archived brain...