10月,Broad研究所张锋教授团队在Nature Methods上发表题为“Massively Parallel Single-Nucleus RNA-Seq with DroNc-Seq (doi: 10.1038/nmeth.4407)” 的文章,提出了一个新的测序技术:DroNc-Seq单细胞表达谱分析技术。该技术融合了sNuc-Seq技术与微流控技术的单细胞核RNA测序方法,可以在结构复杂的组织中更有效地分析...
Multimodal single-cell/nucleus RNA sequencing data analysis uncovers molecular networks between disease-associated microglia and astrocytes with implications for drug repurposing in Alzheimer's disease Systematic identification of molecular networks in disease relevant immune cells of the nervous system is ...
as single-cell methods are lossy, the quantification of snuclRNA-seq produces sparse data matrices, and the expression of each gene is not detected for each nucleus. The structure of the data complicates the clustering of the nuclei into distinct...
Single-nucleus RNA-seq interrogation of the human kidney.aOverview of kidney tissue processing methods tested for nuclei isolation and snDrop-seq.bUMAP visualization of 17,659 single nuclei (15 individuals, Supplementary Data4) passing QC filtering (Supplementary Fig.2a). Source data are provided a...
Single-cell RNA-seq analysis reveals that immune cells induce human nucleus pulposus ossification and degenerationdoi:10.3389/fimmu.2023.1224627NUCLEUS pulposusOSSIFICATIONRNA sequencingGENE expression profilingINTERVERTEBRAL diskBackground and aims: Determining the transcriptomes and mol...
Decoding neuronal genes in stroke-induced pain: insights from single-nucleus sequencing in mice Yan Niu Xiaoping Chen Tianfeng Huang BMC Neurology (2024) Multi-omics analysis reveals NNMT as a master metabolic regulator of metastasis in esophageal squamous cell carcinoma Qi Huang Haiming Chen Xia...
Using single nuclei for RNA-seq to capture the transcriptome of postmortem neurons A protocol is described for sequencing the transcriptome of a cell nucleus. Nuclei are isolated from specimens and sorted by FACS, cDNA libraries are constructed and RNA-seq is performed, followed by data analysis....
10月,Broad研究所张锋教授团队在Nature Methods上发表题为“Massively Parallel Single-Nucleus RNA-Seq with DroNc-Seq (doi: 10.1038/nmeth.4407)” 的文章,提出了一个新的测序技术:DroNc-Seq单细胞表达谱分析技术。该技术融合了sNuc-Seq技术与微流控技术的单细胞核RNA测序方法,可以在结构复杂的组织中更有效地分析...
However, there is a fundamental gap in knowledge for the EAT's role in health and disease.#Methods:We employed single-nucleus RNA sequencing (snRNAseq... A Viveiros,KJ Brown,H Chen,... - 《Circulation》 被引量: 0发表: 2023年 METHODS FOR DETERMINING SPATIAL AND TEMPORAL GENE EXPRESSION ...
We perform single-cell RNA sequencing analysis of 1916 individual cells from eight organs and tissues of E9.5 to E11.5 mouse embryos, namely, the forebrain, hindbrain, skin, heart, somite, lung, liver, and intestine. Based on the regulatory activities rather than the expression patterns, all ...