10月,Broad研究所张锋教授团队在Nature Methods上发表题为“Massively Parallel Single-Nucleus RNA-Seq with DroNc-Seq (doi: 10.1038/nmeth.4407)” 的文章,提出了一个新的测序技术:DroNc-Seq单细胞表达谱分析技术。该技术融合了sNuc-Seq技术与微流控技术的单细胞核RNA测序方法,可以在结构复杂的组织中更有效地分析...
Multimodal single-cell/nucleus RNA sequencing data analysis uncovers molecular networks between disease-associated microglia and astrocytes with implications for drug repurposing in Alzheimer's disease Systematic identification of molecular networks in disease relevant immune cells of the nervous system is ...
This investigation used single-nucleus RNA sequencing technology to identify cell types from liver tissues of pigs, providing a theoretical basis for further investigating liver cell types in pigs. Results: The analysis revealed 13 cells clusters which were further identified 7 cel...
Syncytial skeletal muscle cells contain hundreds of nuclei in a shared cytoplasm. We investigated nuclear heterogeneity and transcriptional dynamics in the uninjured and regenerating muscle using single-nucleus RNA-sequencing (snRNAseq) of isolated nucle
Single-cell RNA sequencing (scRNA-seq) provides valuable insights into human islet cell types and their corresponding stable gene expression profiles. However, this approach requires cell dissociation that complicates its utility in vivo. On the other ha
The small molecule inhibitor Pitstop2 (scip-ATAC-seq) [11] improves the efficiency of transposase entry into the nucleus and thus enhances library complexity and resolution. Transcript-indexed ATAC-seq (T-ATAC-seq) [12] using microfluidic devices allows sequencing of T cell receptor-encoding genes...
Here, we perform single-nucleus RNA-sequencing (snRNA-seq) on nearly 300,000 nuclei from the parietal cortex of AD autosomal dominant (APP and PSEN1) and risk-modifying variant (APOE, TREM2 and MS4A) carriers. Within individual cell types, we capture genes commonly dysregulated across variant...
Neuronal subtypes and diversity revealed by single-nucleus RNA sequencing of the human brain. Science. 2016;352(6293):1586–90. Article CAS Google Scholar 10XGenomics. Genomics X: isolation of nuclei for single cell RNA sequencing. Qiu X, Mao Q, Tang Y, Wang L, Chawla R, Pliner HA, ...
Single-nucleus RNA sequencing (sNuc-RNAseq) is an emerging powerful genomics technology that combines droplet microfluidics with next-generation sequencing to interrogate transcriptome changes at single nucleus resolution. Here we developed Abacus, a flexible UMI counter software for sNuc-RNAseq analysis....
Aprotocolisdescribedforsequencingthetranscriptomeofacellnucleus.Nucleiareisolated fromspecimensandsortedbyFACS,cDNAlibrariesareconstructedandRNA-seqisperformed, followedbydataanalysis.Somestepsfollowpublishedmethods(Smart-seq2forcDNAsynthesis andNexteraXTbarcodedlibrarypreparation)andarenotdescribedindetailhere.Previous...