Using single nuclei for RNA-seq to capture the transcriptome of postmortem neurons A protocol is described for sequencing the transcriptome of a cell nucleus. Nuclei are isolated from specimens and sorted by FACS, cDNA libraries are constructed and RNA-seq is performed, followed by data analysis....
Here, we applied single-nucleus RNA sequencing to characterize the roots of Medicago truncatula at 30 min, 6 h and 24 h after nod factor treatment. We found drastic global gene expression reprogramming at 30 min in the epidermis and cortex and most of these changes were restored at...
As a necessary step in gaining a deeper understanding of the regulatory mechanisms controlling plant gene expression, we report the use of single-nucleus RNA sequencing (sNucRNA-seq) and single-nucleus assay for transposase accessible chromatin sequencing (sNucATAC-seq) technologies on Arabidopsis ...
We also describe the current status of bioinformatics tools used to analyze single-cell RNA-seq datasets and how additional emerging technologies such as spatial transcriptomics and long-read sequencing technologies will provide additional information on the differential use of the genome by plant cells....
题目: Div-Seq:单核RNA-Seq揭示了罕见的成年新生神经元的动态 DOI:https://doi.org/10.1126/science.aad7038 Cite: Habib N, Li Y, Heidenreich M. et al. Div-Seq: Single-nucleus RNA-Seq reveals dynamics of rare adult newborn neurons.Science. 2016 Aug 26;353(6302):925-8. ...
从机制上讲,CTCs的单细胞RNA测序分析显示,在患者和小鼠模型中,有丝分裂基因仅在静止期阶段出现明显的上调,从而实现转移熟练程度。系统地,我们发现关键的昼夜节律激素,如褪黑激素,睾酮和糖皮质激素决定了CTC的产生动力学,因此,胰岛素直接促进肿瘤细胞在体内增殖,但以时间依赖的方式。因此,具有高转移倾向的CTCs的自发...
Defining cellular and molecular identities within the kidney is necessary to understand its organization and function in health and disease. Here we demonstrate a reproducible method with minimal artifacts for single-nucleus Droplet-based RNA sequencing
Specifically, we develop a network-based methodology that leverages single-cell/nucleus RNA sequencing data from both transgenic mouse models and AD patient brains, as well as drug-target network, metabolite-enzyme associations, the human protein-protein interactome, and large-scale longitudinal patient...
Here, we develop a method for obtaining single-nucleus suspensions from snap-frozen human heart tissue for use in DroNc-seq, expanding the variety of ... S Lozano - 《Protocols Io》 被引量: 0发表: 2018年 Single-Nucleus RNA Sequencing, Droplet by Droplet Called DroNc-Seq, it merges an ea...
First, we used single-nucleus RNA sequencing to estimate RNA velocity. Compared to single-cell sequencing, snRNA-seq is more amenable to transcriptomic profiling of postmortem samples because isolated nuclei are intact in frozen tissues29. Moreover, dissociating whole cells from the brain is ...