Nanogrid single-nucleus RNA sequencing reveals phenotypic diversity in breast cancer Single cell RNA sequencing has emerged as a powerful tool for resolving transcriptional diversity in tumors, but is limited by throughput, cost and the abi... R Gao,C Kim,E Sei,... - 《Nature Communications》 ...
Here, we applied single-nucleus RNA sequencing to characterize the roots of Medicago truncatula at 30 min, 6 h and 24 h after nod factor treatment. We found drastic global gene expression reprogramming at 30 min in the epidermis and cortex and most of these changes were restored at...
As a necessary step in gaining a deeper understanding of the regulatory mechanisms controlling plant gene expression, we report the use of single-nucleus RNA sequencing (sNucRNA-seq) and single-nucleus assay for transposase accessible chromatin sequencing (sNucATAC-seq) technologies on Arabidopsis ...
We also describe the current status of bioinformatics tools used to analyze single-cell RNA-seq datasets and how additional emerging technologies such as spatial transcriptomics and long-read sequencing technologies will provide additional information on the differential use of the genome by plant cells....
Here, we perform single-nucleus RNA-sequencing (snRNA-seq) on nearly 300,000 nuclei from the parietal cortex of AD autosomal dominant (APP and PSEN1) and risk-modifying variant (APOE, TREM2 and MS4A) carriers. Within individual cell types, we capture genes commonly dysregulated across variant...
题目: Div-Seq:单核RNA-Seq揭示了罕见的成年新生神经元的动态 DOI:https://doi.org/10.1126/science.aad7038 Cite: Habib N, Li Y, Heidenreich M. et al. Div-Seq: Single-nucleus RNA-Seq reveals dynamics of rare adult newborn neurons.Science. 2016 Aug 26;353(6302):925-8. ...
Single-nucleus RNA and ATAC sequencing reveals the impact of chromatin accessibility on gene expression inArabidopsisroots at the single-cell level Andrew Farmer, Sandra Thibivilliers, Kook Hui Ryu, John Schiefelbein, Marc Libault 1 March 2021 ...
Defining cellular and molecular identities within the kidney is necessary to understand its organization and function in health and disease. Here we demonstrate a reproducible method with minimal artifacts for single-nucleus Droplet-based RNA sequencing
Specifically, we develop a network-based methodology that leverages single-cell/nucleus RNA sequencing data from both transgenic mouse models and AD patient brains, as well as drug-target network, metabolite-enzyme associations, the human protein-protein interactome, and large-scale longitudinal patient...
A 3D gene expression atlas of the floral meristem based on spatial reconstruction of single nucleus RNA sequencing data Article Open access 20 May 2022 Introduction Many dicotyledonous plants such as Arabidopsis have a root system composed of a single primary root and numerous lateral roots. By co...