Our nuclei isolation procedure can be applied in different plant species and tissues, thus expanding the toolkit for plant single-cell genomics experiments. SIGNIFICANCE STATEMENT We introduce an optimized plant nuclei isolation procedure followed by single nuclei RNA-seq that can be applied to ...
RNA sequencing (RNA-seq) emerged from the advent of NGS technologies in the mid-2000s. It provided a snapshot of the transcriptome at a specific moment, enabling researchers to investigate a cell’s function, state, and response to its environment at that time. Crucially, it also operated a...
Single-nucleus RNA sequencing (sNucRNA-seq) is an emerging technology that has been rapidly adopted and demonstrated to be a powerful tool for detailed characterization of each cell- and sub cell-types in complex tissues of higher eukaryotes. sNucRNA-seq has also been used to dissect cell-type...