CELL nucleiGENE expressionFROZEN human embryosFROZEN semenWhether single-cell RNA-sequencing (scRNA-seq) captures the same biological information as single-nucleus RNA-sequencing (snRNA-seq) remains uncertain and likely to be context-dependent. Herein, a head-to-head com...
Single cell RNA-sequencing (scRNASeq) has advanced our understanding of lung biology, but utility is limited by the need for fresh samples, loss of cell types by death or inadequate dissociation, and transcriptional stress responses induced during tissue digestion. Single nucleus RNASeq (snRNASeq)...
Single-cell genomics is essential to chart tumor ecosystems. Although single-cell RNA-Seq (scRNA-Seq) profiles RNA from cells dissociated from fresh tumors, single-nucleus RNA-Seq (snRNA-Seq) is needed to profile frozen or hard-to-dissociate tumors. Each
Single-cell RNA-seq has become routine for discovering cell types and revealing cellular diversity, but archived human brain samples still pose a challenge to current high-throughput platforms. We present STRT-seq-2i, an addressable 9600-microwell array platform, combining sampling by limiting diluti...
Single Cell RNA-seq Neuroscience Before you begin Preparing to use the scREAD website Timing: 5 min 1. We recommend using Chrome, Safari, Microsoft Edge, or Firefox web browser to access scREAD (https://bmbls.bmi.osumc.edu/scread/). Microsoft Internet Explorer is not supported. ...
RNA-seq: RNA-sequencing ROC: Receiver operating characteristic SPT: Sucrose preference test SZ: Schizophrenia scRNA-seq: Single-cell RNA sequencing References GBD. Global, regional, and national burden of 12 mental disorders in 204 countries and territories, 1990–2019: a systematic analysis...
Single nucleus RNA-Seq (sNuc-Seq) profiles RNA from tissues that are preserved or cannot be dissociated, but does not provide the throughput required to analyse many cells from complex tissues. Here, we develop DroNc-Seq, massively parallel sNuc-Seq with droplet technology. We profile 29,543 ...
in the context of PD have not yet been described. Recent technological advancements have facilitated more granulated analyses on the molecular phenotypes of PD at the single-cell level. Single-nucleus RNA sequencing (snRNA-seq) studies have examined cellular heterogeneity of the substantia nigra (SN...
Massively parallel single-cell and single-nucleus RNA sequencing has opened the way to systematic tissue atlases in health and disease, but as the scale of data generation is growing, so is the need for computational pipelines for scaled analysis. Here we developed Cumulus—a cloud-based framework...
DroNc-seq enables low-cost, high-throughput single-nucleus RNA-seq of tissues that are archived or difficult to dissociate, such as post-mortem human brain. Single-nucleus RNA sequencing (sNuc-seq) profiles RNA from tissues that are preserved or cannot b