Here, using deep sequencing of nuclear RNAs (nucRNA-Seq) in parallel with chromatin immunoprecipitation sequencing (ChIP-Seq) of active RNA polymerase II, we compared the nuclear transcriptome of mouse anemic spleen erythroid cells with polymerase occupancy on a genome-wide scale. We demonstrate ...
RNA-sequencing (snRNA-seq) methods, here we show that snRNA-seq faithfully recapitulates transcriptional patterns associated with experience-driven induction of activity, including immediate early genes (IEGs) such asFos,ArcandEgr1. SnRNA-seq of mouse dentate granule cells reveals large-scale changes ...
S. Splicing kinetics and coordination revealed by direct nascent RNA sequencing through nanopores. Mol. Cell 77, 985–998.e8 (2020). Article CAS PubMed Google Scholar Sousa-Luís, R. et al. POINT technology illuminates the processing of polymerase-associated intact nascent transcripts. Mol. ...
RNA ribonucleic acid: any of a class of single-stranded nucleic acid molecules of ribose and uracil, found chiefly in the cytoplasm of cells and in certain viruses; important in protein synthesis and in the transmission of genetic information transcribed from DNA. Comparemessenger RNA,ribosomal RNA...
Compared to standard RNA-seq, microRNA sequencing captures small RNAs enriched by size selection. In parallel to mRNA sequencing, microRNA sequencing has advantages in identifying novel microRNA and detecting microRNA variants over methods of hybridization-based arrays or RT-PCR. Currently, 1600 ...
RNA sequencing (RNA-seq) reads from globular and heart-stage mutant embryos uncovered splicing defects sorted into the major four categories: intron retention (IR), alternative 5′ or 3′ splice site (5′SS or 3′SS), and exon skipping (ES) (Figure S5A; Table S2). The distribution of ...
A rapid method for the isolation and purification of both cytoplasmic and nuclear RNA from cultured animal cells and small tissue samples.
Partial characterization of these RNAs, based on cell fractionation, polyacrylamide gel electrophoretic and chemical sequencing techniques, as well as comparison with literature data, revealed that, in addition to tRNA, 5S RNA and 5.8S RNA, plant nuclei contain two families of low-molecular-mass ...
Although the protein- coding gene regions often had a higher percent of correct identification compared with ribosomal markers, low PCR amplification and sequencing success eliminated them as candidates for a universal fungal barcode. Among the regions of the ribosomal cistron, the internal transcribed ...
we show by single nuclear RNA-sequencing of cardiomyocytes from both mouse and human failing, and non-failing adult hearts that sub-populations of cardiomyocytes upregulate cell cycle activators and inhibitors consequent to the stress-response in vivo. We characterize these subgroups by weighted gene ...