Here, using deep sequencing of nuclear RNAs (nucRNA-Seq) in parallel with chromatin immunoprecipitation sequencing (ChIP-Seq) of active RNA polymerase II, we compared the nuclear transcriptome of mouse anemic spleen erythroid cells with polymerase occupancy on a genome-wide scale. We demonstrate ...
Single-cell RNA-sequencing (RNA-seq) techniques1,2,3,4provide a means to examine the fine-scale differences underlying cell populations that bulk profiling obscures. Due to the high degree of structural and functional heterogeneity that exists between neurons, interest has recently grown to map th...
S. Splicing kinetics and coordination revealed by direct nascent RNA sequencing through nanopores. Mol. Cell 77, 985–998.e8 (2020). Article CAS PubMed Google Scholar Sousa-Luís, R. et al. POINT technology illuminates the processing of polymerase-associated intact nascent transcripts. Mol. ...
The sequencing of cDNA clones representing BC1 RNA showed that this RNA, beginning at the 5’ end, is composed of a sequence complementary to the repetitive ID sequence followed by a poly(A) tract and concluded by a 3’ sequence of 25 nucleotides (DeChiara and Brosius, 1987). The 3’ ...
RNA sequencing (RNA-seq) was used to measure human gene expression levels in EBV-1 (GM12878 & Mutu-III) and EBV-2 (AG876 & Jiyoye) infected cell lines. We generated new RNA-seq datasets for the AG876, Jiyoye, and GM12878 cell lines. Public Mutu-III RNA-seq datasets (see Supplem...
RNA ribonucleic acid: any of a class of single-stranded nucleic acid molecules of ribose and uracil, found chiefly in the cytoplasm of cells and in certain viruses; important in protein synthesis and in the transmission of genetic information transcribed from DNA. Comparemessenger RNA,ribosomal RNA...
To provide further evidence for the cell-type specific maxRNA presentation, we characterized the maxRNA presenting cells by combining isFISH and fluorescence-activated cell sorting (FACS) with single-cell RNA sequencing (scRNA-seq). Specifically, after isFISH labeling, we performed FACS on PBMCs ...
Transcriptional defects and reprogramming barriers in somatic cell nuclear reprogramming as revealed by single-embryo RNA sequencing Yong Liu, Fengrui Wu, Ling Zhang, Xiaoqing Wu, Dengkun Li, Jing Xin, Juan Xie, Feng Kong, Wenying Wang, Qiaoqin Wu, Di Zhang, Rong Wang, Shao...
A rapid method for the isolation and purification of both cytoplasmic and nuclear RNA from cultured animal cells and small tissue samples.
Approximately 26,300 Mb of RNA sequencing (RNA-seq) data obtained under two different culture conditions (seven libraries) were assembled into 63,104 unique transcriptomes (N50 = 1,684 nucleotides), 26,691 of which encoded complete open reading frames (Table 1 and Table S1, parts D and E)...