Nuclear RNA-seq of single neurons reveals molecular signatures of activation. Nat Commun. 2016;7:11022.Lacar B, Linker SB, Jaeger BN, Krishnaswami SR, Barron JJ, Kelder MJ, Parylak SL, Paquola AC, Venepally P, Novotny M, et al: Corrigendum: Nuclear RNA- seq of single neurons reveals...
Single-cell RNA-sequencing (RNA-seq) techniques1,2,3,4provide a means to examine the fine-scale differences underlying cell populations that bulk profiling obscures. Due to the high degree of structural and functional heterogeneity that exists between neurons, interest has recently grown to map t...
we performed several controls. First, we undertook technical replicates of the same nuclear RNA-seq samples using three independent library preparations and found good correlation (r = 0.99) across all three technical replicates (Supplementary Fig.2A–C), reflecting a consistent library preparation...
lncRNA(长链非编码RNA)测序分析服务 面议 产品介绍 单细胞核测序技术是在单细胞水平上发展起来的补足技术,它能够弥单细胞测序的局限性,同时揭示单个细胞的基因结构和基因表达状态,反映细胞间的异质性。 具体来说,两种技术优势对比如下: 单细胞测序 单细胞核测序 冻存组织 细胞活性丧失,死细胞无法进行scRNA-seq 细胞...
RNA-seq and data analysis Total RNA was isolated from the NC, NONO-knockdown, and PKM2-knockdown MDA-MB-231 cells. RNA purification, reverse transcription, library construction, and sequencing were performed in collaboration with Majorbio Biotechnology (Shanghai, China) according to the manufacturer’...
优异的细胞质和核RNA分离和纯化;方便快捷的旋转柱形式;高质量和高产量的RNA;在没有苯酚的情况下分离完整多样性的RNA(包括microRNA);纯化的RNA可用于任何应用,包括RT-PCR,qRT-PCR,RNA-Seq,微阵列等;细胞质RNA不含DNA,可直接用于RT-PCR / qRT-PCR。
Why is the RNA degraded? Why is the RNA not performing well in downstream applications? I noticed genomic DNA contamination in cytoplasmic fraction. How to prevent this? What are suggested markers to differentiate between the cytoplasmic and nuclear fractions using RT-PCR?
Paired-end 150-bp sequencing was further performed on a HiSeq 2500 (Illumina) platform at the Annoroad Gene Technology Corporation, Ltd. (Beijing, China). Analysis of the RNA-seq data A Perl script was used to filter the original data (raw data) and guarantee the quality of the data for...
Similarly, RNA-seq can be used to profile small non-codingRNA, such asmicroRNAs(miRNAs) in cancers. miRNAs are short(20–24 nucleotides)non-coding RNAs that modulateprotein translationandmRNA stabilityin a tissue-specific manner and regulate proliferation, apoptosis, differentiation, growth, migration...
RNA Mini 96 Kit 2 x 96 preps 6109.00 7326801 Aurum Total RNA Elution Solution 279.00 7326802 Aurum Total RNA Lysis Solution, 85 ml 897.00 7326803 Aurum Total RNA Wash High-Stringency Solution 593.00 7326804 Aurum Total RNA Wash Low-Stringency Solution 637.00 7326805 Aurum DNase Dilution Solution ...