Single-cell RNA-sequencing (RNA-seq) techniques1,2,3,4provide a means to examine the fine-scale differences underlying cell populations that bulk profiling obscures. Due to the high degree of structural and functional heterogeneity that exists between neurons, interest has recently grown to map t...
Here, using deep sequencing of nuclear RNAs (nucRNA-Seq) in parallel with chromatin immunoprecipitation sequencing (ChIP-Seq) of active RNA polymerase II, we compared the nuclear transcriptome of mouse anemic spleen erythroid cells with polymerase occupancy on a genome-wide scale. We demonstrate ...
Single nuclear RNA-seq of left ventricular CMs in vivo Adult CMs are predominantly binucleated and undergo polyploidisation and multi-nucleation during heart failure17. To avoid confounding differences in comparing single cells with different number of nuclei, we reasoned that each single CM nucleus ...
To further understand the EBNA2 type 1 preference for EBF1, we measured EBF1 expression across the EBV type 1 and type 2 cell lines. RNA was extracted from the AG876, Jiyoye, and GM12878 cell lines. We also included Mutu-III RNA-seq data from our previous study [46]. The resulting...
优异的细胞质和核RNA分离和纯化;方便快捷的旋转柱形式;高质量和高产量的RNA;在没有苯酚的情况下分离完整多样性的RNA(包括microRNA);纯化的RNA可用于任何应用,包括RT-PCR,qRT-PCR,RNA-Seq,微阵列等;细胞质RNA不含DNA,可直接用于RT-PCR / qRT-PCR。
Remarkably, only 0.49–3.37% of the nucleomorph genomes of these organisms did not have any mRNA counterpart in our RNA-Seq data sets, and nucleomorph genes show equal or higher levels of transcription than their counterparts in the nuclear genomes. We hypothesize that elevated levels of nucleo...
Gene prediction yielded 41,925 protein models in the ∼616 Mbp assembly, 77.2% of which (32,366 gene models) were supported by RNA-seq data (blastn, e value < 1 × 10−10; Table 1). The vast majority of the transcriptome was encoded in the 616 Mbp draft assembly, suggesting that...
Effective expression of small interfering RNA in human cells In many eukaryotes, expression of nuclear-encoded mRNA can be strongly inhibited by the presence of a double-stranded RNA (dsRNA) corresponding to exon seq... CP Paul,PD Good,I Winer,... - 《Nature Biotechnology》 被引量: 2585...
Excellent separation and purification of cytoplasmic and nuclear RNA Convenient and fast spin column format High quality and yield of RNA Isolate full diversity of RNA (including microRNA) without phenol Purified RNA is ready for any application including RT-PCR, qRT-PCR, RNA-Seq, arrays and more...
To interrogate the functional relevance of maxRNAs in PBMCs, we asked what are the maxRNA-producing genes in these cells. To answer this question, we developed Surface-FISHseq to sequence the isFISH-captured candidate maxRNAs. The central idea of Surface-FISHseq is to purify the cell surface...