Here, using deep sequencing of nuclear RNAs (nucRNA-Seq) in parallel with chromatin immunoprecipitation sequencing (ChIP-Seq) of active RNA polymerase II, we compared the nuclear transcriptome of mouse anemic spleen erythroid cells with polymerase occupancy on a genome-wide scale. We demonstrate ...
Single-cell RNA-sequencing (RNA-seq) techniques1,2,3,4provide a means to examine the fine-scale differences underlying cell populations that bulk profiling obscures. Due to the high degree of structural and functional heterogeneity that exists between neurons, interest has recently grown to map th...
Single nuclear RNA-seq of left ventricular CMs in vivo Adult CMs are predominantly binucleated and undergo polyploidisation and multi-nucleation during heart failure17. To avoid confounding differences in comparing single cells with different number of nuclei, we reasoned that each single CM nucleus ...
To further understand the EBNA2 type 1 preference for EBF1, we measured EBF1 expression across the EBV type 1 and type 2 cell lines. RNA was extracted from the AG876, Jiyoye, and GM12878 cell lines. We also included Mutu-III RNA-seq data from our previous study [46]. The resulting...
Surface-seq: characterization of maxRNAs by sequencing To inspect the possible cell surface-presentation of nuclear-encoded RNA, we developed a technology called Surface-seq. Surface-seq is based on a nanotechnology that extracts the plasma membrane from cells and tightly assembles the membrane around...
优异的细胞质和核RNA分离和纯化;方便快捷的旋转柱形式;高质量和高产量的RNA;在没有苯酚的情况下分离完整多样性的RNA(包括microRNA);纯化的RNA可用于任何应用,包括RT-PCR,qRT-PCR,RNA-Seq,微阵列等;细胞质RNA不含DNA,可直接用于RT-PCR / qRT-PCR。
RNA-sequencing showed that 8.5% of TNF-± transcriptional responses were TonEBP-dependent and identified genes regulated by both TNF-± and TonEBP. These genes were over-enriched in pathways and diseases related to Inflammatory Response and Inhibition of Matrix Metalloproteases. Based on RNA-seq ...
RNA-seq analysis was also performed with GAIIx. A set of gene model predictions (the Symbiodinium minutum Gene Model version 1.2) was generated mainly with AUGUSTUS, and a genome browser has been established with the Generic Genome Browser 2.17, as employed in our previous studies [25, 69]....
Excellent separation and purification of cytoplasmic and nuclear RNA Convenient and fast spin column format High quality and yield of RNA Isolate full diversity of RNA (including microRNA) without phenol Purified RNA is ready for any application including RT-PCR, qRT-PCR, RNA-Seq, arrays and more...
Document S1. Figures S1–S5 and Table S2 Table S1. RNA-Seq Analysis of the mas1 Mutant versus Wild-Type Strain at Permissive Temperature and after Shift to Non-permissive Temperature for 2 and 4 h, Respectively, Related to Figure 2 Document S2. Article plus Supplemental InformationVolume...