No matter how you are using your cells, you can count on these products to help keep them healthy. GROW Gibco cell culture media & reagents Gibco growth factors Gibco FBS Countess II FL Automated Cell Counter MODIFY Lipofectamine transfection reagents Neon electroporation transfection system ...
2. 细胞株的来源很重要,如直接来源于ATCC(American Type Culture Collection 美国模式菌种保藏中心)生命力要强于国内细胞库的。 3. 注意细胞的密度一定要高,介于5×10^5—— 10^6/mL之间,增值的快。细胞数量太少,不适合THP-1生长,一般状态不好的THP-1在培养了3天后数量仍然不多,继续培养一天或者两天,培养基...
give you confidence in the health of your cells every step of the way, we’ve highlighted the technologies and products within cell biology that are critical to maintaining optimal cell health. No matter how you are using your cells, you can count on these products to help keep them healthy...
Adenocarcinoma解冻后须较高之浓度,而HeLa只需1～3×106cells/ml ④other suspensions:5～10×106cells/ml,human lymphocyte须至少5×106cells/ml。 (3)冷冻保护剂浓度为5或10%DMSO,若是不确定细胞之冷冻条件,在做冷冻保存之同时,亦应作一个backup culture,以防止冷冻失败。 (4)DMSO(二甲基亚砜)在常温下对细胞...
根据传统定义,次收获和接种的细胞被称为原代细胞[Freshney, R.I. (1987). Culture of Animal Cells. A Manual of Basic Technique. (New York, Alan R. Liss, Inc.)].这类细胞生命周期有限,在有限的生命周期内需掌握HAO细胞大的生长空间,以保持细胞群中基因型和表型的一致性。细胞系是不断生长,分化的细胞...
As a result, THP1-Blue™ NF-κB cells allow the monitoring of NF-κB activation by assessing the activity of SEAP (secreted embryonic alkaline phosphatase). The level of NF-κB-induced SEAP in the cell culture supernatant is readily assessed with QUANTI-Blue™, a SEAP detection reagent. ...
保存条件:液氮保存纯度规格:1x10(6)viable cells/ml 产品类别:实验化学品 "THP-1 Cell:人单核细胞白血病细胞系 细胞形态:淋巴母细胞样 细胞生长:悬浮 细胞背景资料:该细胞从一名1岁的患有急性单核细胞性白血病的男孩的外周血中分离建立。该细胞可以吞噬乳胶颗粒和激活的红细胞,细胞膜和胞浆内均没有免疫球蛋白,...
方法/步骤 1 细胞培养：THP-1细胞培养于含有10%FBS+1%P/S的RPMI-1640培养基中，培养环境为37℃，5%CO2。可以每隔3-4天加一些新鲜的培养基，或直接传代。2 细胞传代：当细胞密度达到约8x10^5cells/ml左右时，即可进行传代。由于是悬浮细胞，可直接将细胞吸出，离心之后传代。传代最少浓度不应少于1x10^5 ...
Culture-derived microparticles engineered to contain PPARγ are internalized by THP-1 cells.Julie SahlerCollynn F. WoellerRichard P. Phipps
THP-1是从急性单核细胞白血病患者的外周血中分离出的单核细胞。在某些诱导分化剂作用下,THP-1细胞可以被诱导分化为多种巨噬细胞,是研究炎症、免疫反应等领域的理想模型。此外,还可使用CRISPR Cas9技术编辑的THP-1细胞进行巨噬细胞作用机制、免疫应答通路研究、炎症疾病治疗开发等研究。