We also present a step-by-step protocol for a transwell co-culture using a human breast cancer cell line, HCC1806, and THP-1-derived macrophages. Finally, we describe the colony formation and migration assays performed on the breast cancer cells after the co-culture with macrophages to ...
询价 索取Protocol 产品描述 产品数据库I. Background STING(干扰素基因刺激因子)又称 MPYS、TMEM173、MITA 和 ERIS,是一种跨膜蛋白,是环状二核苷酸(CDN)的直接传感器。CDN 是细菌信号转导中普遍存在的第二信使分子,是哺乳动物细胞的防御触发器。当细菌病原体攻击时,释放到细胞中的 CDN 直接与 STING 结合,激活...
Lipofectamine® LTX 试剂是一种专有的无动物源性制剂,其细胞毒性较低,适用于将 DNA 转染到真核细胞中。本参考资料提供了使用 Lipofectamine® LTX 试剂(货号 15338-100)将质粒 DNA 转染至 THP-1 人单核细胞性白血病细胞(ATCC 货号 TIB-202)中的推...
Moore GE et al (1967) Culture of normal human leukocytes. JAMA 199:519–524 Article CAS PubMed Google Scholar Moreno-Navarrete JM, Ortega FJ et al (2009) Decreased circulating lactoferrin in insulin resistance and altered glucose tolerance as a possible marker of neutrophil dysfunction in type...
The ADCP Reporter Bioassay Effector Cells (THP-1) are provided in thaw-and-use format as cryopreserved cells that can be thawed, plated and used in an assay without the need for cell culture and propagation. When cocultured with a target cell and relevant antibody, the ADCP Reporter Bioassay...
RPMI 1640, 2 mM L-glutamine, 25 mM HEPES, 10% heat-inactivated fetal bovine serum (30 min at 56 °C), 100 μg/ml Normocin™, Pen-Strep (100 U/ml-100 μg/ml) Initial culture of all THP-1 derived cells must be performed in growth medium containing 20% heat-inactivated FBS. ...
utilising VD 3and PMA in comparison to primary human monocytes or monocyte-derived macrophages (MDM).Both stimuli induced changes in cell morphology indicative of differentiation but neither showed differentiation comparable to MDM.In contrast,PMA treatment followed by 5days resting in culture without ...
Protocol 1. Suspend THP-1 monocytic cells at a concentration of 5 × 105 cells/mL in Culture media and aliquot 200 μL to each well of a flat-bottom tissue culture treated 96-well plate. 2. Add PMA to a final concentration of 50 nM. 3. Incubate plates at 37 °C, 5% CO2 for 16...
After solar radiation (1.7 mW·cm−2, 50 min) or non-radiation treatment, cells were placed into the incubator for further culture for 5 h. Photostimulant should be pre-irradiated (30 min of light, then 15 min of avoid light) before formal light exposure. The contents of cytokines IL-...
比较不同诱导条件下的细胞THP-1细胞