THP1-Dual™ cells are resistant to the selectable markers blasticidin and Zeocin ®. SAFETY CONSIDERATIONS Biosafety Level 1 HANDLING PROCEDURES Required Cell Culture Medium • Growth Medium: RPMI 1640, 2 mM L-glutamine, 25 mM HEPES, 10% heat-inactivated fetal bovine serum (30 min at ...
2-4 mM L-glutamine, 10% (v/v) heat‑inactivated fetal bovine serum (FBS; 30 min at 56 ºC), 50 U/ml penicillin, 50 mg/ml streptomycin, 100 mg/ml NormocinTMNotes:- The use of NormocinTM together with Pen-Strep is required to keep the cells free of microbial contaminants. Co...
产品名称: THP1-Dual? Cells .0 订购此产品 供应商: Invivogen 规格: 3-7 x 10e6 目录价: 16507.5 库存状态: 三周到货 CAS编号: 应用范围: 生化试验 种属来源: 相关信息: THP1-Dual™ Cells THP1-Dual™ cells were derived from the human THP-1 monocyte cell line by stable integration...
Monocytes are circulatory precursor cells from myeloid origin that can develop into macrophages or dendritic cells upon migration from the blood stream to tissues. Both macrophages and dendritic cells are professional antigen-presenting cells. Monocytes
Compared with the control group, THP-1 cells in the miR-126-3p overexpression group had low proliferative capacity after 48 h and 72 h of culture (both P < 0.05), and the cells were blocked in G 1 phase [proportion of cells in G 1 phase: (58.2±2.8)% vs. (44.1±2.4)%, P < ...
Monocytes are circulatory precursor cells from myeloid origin that can develop into macrophages or dendritic cells upon migration from the blood stream to tissues. Both macrophages and dendritic cells are professional antigen-presenting cells. Monocytes
2.5 Co-cultivation of THP-1 cells with other cell types Apart from studying responses of THP-1 cells in the monocyte or macrophage state as a single cell type, this cell model has been used in co-culture system with other cells, for instance, vascular smooth muscle cells, adipocytes, T-...
Culture, stimulation and harvesting of THP-1 cells Human THP-1 cells were grown in RPMI 1640 medium containing 10% fetal bovine serum (FBS), 2 mM glutamine, 10 mM HEPES, 1 mM sodium pyruvate, 561025 M b-mercaptoethanol, penicillin (100 U/mL), and streptomycin (100 mg/mL) and stored ...
Macrophages are the main host cells of Mtb and their apoptosis, necrosis, autophagy and inflammation are essential components of the host defense against Mtb22. BCG can induce macrophage apoptosis and inflammation by activation of MAPKs/NF-κB signaling pathway23,24,25, and DUSP1 is a dual-spec...
In addition, HSV-1 infects mono- cytic cells, such as human leukemic monocytic lymphoma (U937) cells or human acute monocytic leukemia (THP-1) cells, with different degrees of permissiveness19. Freshly isolated or non-activated monocytic cells are resistant to HSV-1 infection20. In contrast,...