2-4 mM L-glutamine, 10% (v/v) heat‑inactivated fetal bovine serum (FBS; 30 min at 56 ºC), 50 U/ml penicillin, 50 mg/ml streptomycin, 100 mg/ml NormocinTMNotes:- The use of NormocinTM together with Pen-Strep is required to keep the cells free of microbial contaminants. Co...
As a result, THP1-Dual™ cells allow the simultaneous study of the NF-κB pathway, by monitoring the activity of SEAP, and the IRF pathway, by assessing the activity of a secreted luciferase, Lucia luciferase. Both reporter proteins are readily measurable in the cell culture supernatant when...
2.5 Co-cultivation of THP-1 cells with other cell types Apart from studying responses of THP-1 cells in the monocyte or macrophage state as a single cell type, this cell model has been used in co-culture system with other cells, for instance, vascular smooth muscle cells, adipocytes, T-...
Monocytes are circulatory precursor cells from myeloid origin that can develop into macrophages or dendritic cells upon migration from the blood stream to tissues. Both macrophages and dendritic cells are professional antigen-presenting cells. Monocytes
Cells were seeded onto 6-well plates at a density of 5×105 cells/well and cultured overnight in an incubator. After the cells were covered with the culture plate, the tip of a 20 µl pipette gun was used to scratch the cell monolayer perpendicular to the plate, with an interval of...
Macrophages are the main host cells of Mtb and their apoptosis, necrosis, autophagy and inflammation are essential components of the host defense against Mtb22. BCG can induce macrophage apoptosis and inflammation by activation of MAPKs/NF-κB signaling pathway23,24,25, and DUSP1 is a dual-spec...
Culture was carried out at 37°C in a humidified incubator containing 5% CO2, and the medium was refreshed every 2–3 days. For the experiment, the cells were seeded at a density of 1.0×105 cells per milliliter and were differentiated into macrophages by adding 100 ng...
Aβ peptides, if used in the indicated concentrations in the presence of TGF-β1, did not significantly affect the viability of THP-1 cells when LDH release was measured. The effects of Me2SO alone in the used range of concentrations were not detectable. Cell Culture and Treatment for ...
6. Transfer the cells to a T-25 culture flask containing 5 ml of growth medium (with 20% heat-inactivated FBS). 7. Place the culture at 37 °C in 5% CO2. REPORTER ASSAY To ensure the best results, use THP1-Dual™ hTLR9 cells with less than 20 passages. Cell preparation...
α7 nicotinic acetylcholine receptors (nAChRs) are ubiquitous in the nervous system and ensure important neurophysiological functionality for many processes. However, they are also found in cells of the immune system, where their role has been less studi