Subculturing: Protocol: Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 2-4 X 10(5) viable cells/ml. Subcultutre when cell concentration reaches 8X105) cells/ml.Do ...
PBMC-derived monocytes is often limited and these cells cannot be stocked in liquid nitrogen. THP-1 cells can be stored for a number of years and by following an appropriate protocol, this cell line can be recovered without any obvious effects on monocyte–macrophage features and cell viability...
比较不同诱导条件下的细胞THP-1细胞
As THP-1 and U937 are very frequently used, we focus here on these two cell lines. 5 General Protocol of Culturing THP-1 Cells Roswell Park Memorial Institute (RPMI) 1640 medium is a commonly used medium for THP-1 as well as for U937 cells. Alternatively, DMEM (Dulbecco’s Modified ...
6. Add 20 μl of stimulated THP1-Dual™ hTLR9 cell culture supernatant per well. 7. Incubate the plate at 37 °C for 1-3 h. 8. Determine SEAP levels using a spectrophotometer at 620-655 nm. Detection of IRF induction Below is a protocol for end-point readings using ...
We also present a step-by-step protocol for a transwell co-culture using a human breast cancer cell line, HCC1806, and THP-1-derived macrophages. Finally, we describe the colony formation and migration assays performed on the breast cancer cells after the co-culture...
THP-1 cell culture and cell treatmentHuman THP-1 cells (West China School of Preclinical and Forensic Medicine, Sichuan University, China), a human monocytic leukemia cell line, were cultured in RPMI-1640 medium containing 15% (v/v) fetal calf serum in a humidified atmosphere of 5% CO2 at...
Lipofectamine® LTX 试剂是一种专有的无动物源性制剂,其细胞毒性较低,适用于将 DNA 转染到真核细胞中。本参考资料提供了使用 Lipofectamine® LTX 试剂(货号 15338-100)将质粒 DNA 转染至 THP-1 人单核细胞性白血病细胞(ATCC 货号 TIB-202)中的...
Cell culture, sonodynamic therapy protocol Human THP-1 monocytes were purchased from American Type Culture Collection (Manassas, VA, USA) and cultured as previously described.14For experiments, the cells at logarithmic growth phase were seeded at 1.0 × 105cells/ml in 100 ng/ml phorbol-12-myri...
Cell culture Human myeloid leukemia mononuclear cells THP-1 and mouse macrophage-like (RAW264.7) cell were obtained from the Cell Bank of the Chinese Academy of Sciences (Shanghai, China). THP-1 cells were cultured in RPMI 1640 medium (Gibco, USA) supplemented with 10% fetal bovine serum (...