Protocol for primary T cell culture displays phenotypic markers and transcriptional changes indicative of activation.Thomas, C. WhisenantEigen, R. PeraltaLauren, D. AarrebergNina, J. GaoSteven, R. HeadPhillip, OrdoukhanianJamie, R. Williamson...
Cell culture and the eukaryotic cells used in biotechnology W T. Godbey, in Biotechnology and its Applications (Second Edition), 2022 7.2.1 Primary cells Cells that have been taken directly from a body or a tissue are known as primary cells, and they can be obtained by biopsy, surgery,...
T cells expressing synthetic receptors. Functional assays for quantifying the ability of CAR T cells to sense and respond to soluble ligands are also presented. This protocol provides a framework for proficient immune-cell researchers to test novel T-cell therapies targeting soluble ligands in <2 ...
Integrated culture and purification of rat Schwann cells from freshly isolated adult tissue We describe a simple, rapid and highly selective protocol for the primary culture of Schwann cells in vitro from freshly dissociated adult rat nerve. The p... R Kaewkhaw,AM Scutt,JW Haycock - 《Nature...
MJ Heukels-Dully,MF Niermeijer - 《Experimental Cell Research》 被引量: 83发表: 1976年 Two-stage culture protocol for isolating mesenchymal stem cells from amniotic fluid A method of harvesting mesenchymal stem cells from human amniotic fluid uses a two-stage culture protocol comprising culturing ...
However, to limit the potential impact on extra processing time on cell viability, given the high numbers of immune cells in mesenteric WAT, we opted to exclude the Percoll gradient step from our protocol. Of note, the autofluorescence of cells derived from liver and mesenteric WAT tissues may...
32. The goal of this experiment was to build on these previous cnidarian cell culture studies and produce reliable cell cultures that can be used as a versatile tool for live cell assays. We developed an extensive antimicrobial pre-treatment protocol to reduce microbial contamination. We tested ...
We first optimize an efficient CRISPRi protocol in primary CD4+ T cells via CROPseq vectors. Subsequently, we perform a screen targeting 45 non-coding regulatory elements and 35 transcription start sites and profile approximately 250,000 T -cell single-cell transcriptomes. We develop a bespoke ...
Isolation and culture of hepatic stellate cells from mouse liver. Hepatic stellate cells (HSCs) are the primary extracellular matrix-producing cells within the liver and have numerous vital functions. A robust protocol fo... Wenju,Chang,Mengxuan,... - 《Acta Biochimica Et Biophysica Sinica》 被引...
largely reducing the risk of contamination by other cell types. One other commonly used strategy to expand primary epithelial cells from the cervix is to place the small pieces of explant with mucosal surface down directly into the culture dish11,17,30. In our hands, the present protocol was ...