Johnson MJ, Bunge RP, Wood PM (2001) Primary cell cultures for the study of myelination. In: Fedoroff S, Richardson A (eds) Protocols for neural cell culture, 3rd edn. Humana Press, New Jersey, pp 95-116Johnson MJ, Bunge RP, Wood PM (2001) Primary cell cultures for the study of ...
3.1 Primary cell cultures Primary cell culture is defined as the ex vivo culture of cells freshly obtained from a tissue. The main advantages of primary cells are their faithful transcriptomic and proteomic profile and closer proximity to physiological function and response. Some drawbacks are their ...
Experience in our cell culture laboratories has shown that cells do not suffer deleterious effects if the DMSO concentration is sufficiently low. Therefore, our product instructions include a detailed protocol which involves diluting the cells into culture medium such that the final DMSO concent...
cell in the first 7 days.NGF and BDNF may also be released by the feed layer cells.The NCSC could be induced into neuro-glia cells by the feed layer.Conclusion FBS-free medium could provide a steady condition of Schwann cell growth.The feed layer can release NGF and BDNF steadily and ...
Primary Cells from their source organ tissue and media optimized for each individual cell type (and always in stock).
The final cell preparation was seeded at a density of 105/ml, and about 2.5~5×106 cells were obtained totally. The cells could be well-maintained for 8~10 days by replacing the culture medium every 2 days. The primary cultured hepatocytes could be well subjected to lots of follow-up ...
In light of the lack of information on oenocytes from Aedes aegypti and the potential role of these cells in the biology of this major yellow fever and dengue vector, we developed a protocol to purify and maintain Ae. aegypti pupa oenocytes in primary culture. Ae. aegypti oenocytes were...
In addition to studies of these targets, this protocol can be adapted to characterize other proteins and cell lines using the same modular setup with minor modifications (Fig. 4). Sign in to download hi-res image Fig. 4. Single-molecule imaging setup. (A) Individual laser lines are ...
Isolation and culture of hepatic stellate cells from mouse liver. Hepatic stellate cells (HSCs) are the primary extracellular matrix-producing cells within the liver and have numerous vital functions. A robust protocol fo... Wenju,Chang,Mengxuan,... - 《Acta Biochimica Et Biophysica Sinica》 被引...
Adipose tissueadipose-derived mesenchymal stem cells (ASCs)cell cultureimmunophenotypingmesenchymal stem cells (MSCs)Background: Adipose tissue (AT) is a rich source of mesenchymal stem cells (MSCs), however, there is no standardized protocol for stem cell isolation and culture. This leads to ...