This protocol is using THP-1 cells, a human leukemia monocytic cell line that can be differentiated into macrophages. This allows studying the effects of the macrophage secretome on cancer cells (on e.g. growth, drug response or gene expression) in co-cultures without direct cell contact ...
6. Add 20 μl of stimulated THP1-Dual™ hTLR9 cell culture supernatant per well. 7. Incubate the plate at 37 °C for 1-3 h. 8. Determine SEAP levels using a spectrophotometer at 620-655 nm. Detection of IRF induction Below is a protocol for end-point readings using ...
6. Add 20 µl of THP1-Dual™ cells supernatant. 7. Incubate the plate at 37 °C for 1-6 h. 8. Determine SEAP levels using a spectrophotometer at 620-655 nm. DETECTION OF IFN INDUCTION Below is a protocol for end-point readings using a luminometer with an injector,...
使用PLUS™ 试剂(货号 11514-015)可增强 THP-1 细胞中的转染性能。 我们建议,在络合之前使用 Opti-MEM® I 减血清培养基(货号 31985-062)稀释 DNA 和 Lipofectamine® LTX 试剂。 关于其他专业转染方案(包括关于特定细胞类型的 PLUS™ 试剂和抗...
THP-1 cells can be stored for a number of years and by following an appropriate protocol, this cell line can be recovered without any obvious effects on monocyte–macrophage features and cell viability. 5. The homogeneous genetic background of THP-1 minimizes the degree of variability in the ...
The ADCP Reporter Bioassay Effector Cells (THP-1) are provided in thaw-and-use format as cryopreserved cells that can be thawed, plated and used in an assay without the need for cell culture and propagation. When cocultured with a target cell and relevant antibody, the ADCP Reporter Bioassay...
We also present a step-by-step protocol for a transwell co-culture using a human breast cancer cell line, HCC1806, and THP-1-derived macrophages. Finally, we describe the colony formation and migration assays performed on the breast cancer cells after the co-culture ...
ISRE-Luc/NFκB-SEAP/THP-1CBP74097询价 索取Protocol 产品描述 产品数据库I. Background STING(干扰素基因刺激因子)又称 MPYS、TMEM173、MITA 和 ERIS,是一种跨膜蛋白,是环状二核苷酸(CDN)的直接传感器。CDN 是细菌信号转导中普遍存在的第二信使分子,是哺乳动物细胞的防御触发器。当细菌病原体攻击时,释放到...
a cytokine profile that resembled MDM in response to TLR ligands,in particular with marked TLR2responses.Moreover,both MDM and PMAr retained marked plasticity to stimulus-directed polarization.These findings suggest a modified PMA differentiation protocol can enhance macrophage differentiation of THP-1...
We detail steps for virus production, THP-1 cell infection and clone selection, fixing the suspension cells to the glass slide for immunostaining, and subsequent confocal microscopy. This protocol can be applied to other suspension cells. For complete details on the use and execution of this ...