Required Cell Culture Media* Growth Medium: RPMI 1640, 2-4 mM L-glutamine, 10% (v/v) heat‑inactivated fetal bovine serum (FBS; 30 min at 56 ºC), 50 U/ml penicillin, 50 mg/ml streptomycin, 100 mg/ml NormocinTMNotes:- The use of NormocinTM together with Pen-Strep is ...
Gibco cell culture media & reagents Gibco growth factors Gibco FBS Countess II FL Automated Cell Counter MODIFY Lipofectamine transfection reagents Neon electroporation transfection system Invitrogen RNAi products Recombinant proteins Dynabeads cell separation technology ...
Gibco cell culture media & reagents Gibco growth factors Gibco FBS Countess II FL Automated Cell Counter MODIFY Lipofectamine transfection reagents Neon electroporation transfection system Invitrogen RNAi products Recombinant proteins Dynabeads cell separation technology DETECT Invitrogen imaging reagents Invitrogen...
比较不同诱导条件下的细胞THP-1细胞
The ADCP Reporter Bioassay Effector Cells (THP-1) are provided in thaw-and-use format as cryopreserved cells that can be thawed, plated and used in an assay without the need for cell culture and propagation. When cocultured with a target cell and relevant antibody, the ADCP Reporter Bioassay...
cell morphology indicative of differentiation but neither showed differentiation comparable to MDM.In contrast,PMA treatment followed by 5days resting in culture without PMA (PMAr)increased cytoplasmic to nuclear ratio,increased mitochondrial and lysosomal numbers and altered differentiation-dependent cell ...
It is necessary to rest differentiated THP-1 macrophages at least 24 h in culture media without PMA in order to increase macrophage markers and decrease NF-κB gene clusters which have been up-regulated during differentiation. Cell adherence, high phagocytic capacity and expression of differentiation...
Conditioned media from co-cultured cells were assayed for interleukin (IL)-6, IL-8, prostaglandin E2 (PGE2), PGF2α, and vascular endothelial growth factor (VEGF). Naïve and macrophage-exposed AF cell responses to 10 ng/ml tumor necrosis factor-α (TNF-α) were compared using the same ...
HEPES is a zwitterionic organic chemical buffer and is widely used in many biochemical reactions and as a buffering agent in some cell culture media. These buffers have pK a values between 6.0 and 8.0, high solubility, limited effect on biochemical reactions, membrane permeability, are chemically ...
CCL18 mRNA expression in primary macrophages incubated 16 h with 20 ng/ml of IL-4 (M1:M2 ratio of −19) while 72 h of incubation with IL-4 and IL-13 were required to induce CCL18 expression and to detect a secretion of CCL18 in the culture media of THP-1-derived M2 macrophages...