Frozen Stock Preparation 1. Resuspend cells at a density of 5-7 x 106 cells/ml in freezing medium freshly prepared with cold FBS. Note: A T-75 culture flask yields enough cells for preparing 3-4 frozen vials. 2. Dispense 1 ml of the cell suspension into cryogenic vials. 3. ...
We also present a step-by-step protocol for a transwell co-culture using a human breast cancer cell line, HCC1806, and THP-1-derived macrophages. Finally, we describe the colony formation and migration assays performed on the breast cancer cells after the co-culture ...
THP1-Dual™ cells are resistant to the selectable markers blasticidin and Zeocin ®. SAFETY CONSIDERATIONS Biosafety Level 1 HANDLING PROCEDURES Required Cell Culture Medium • Growth Medium: RPMI 1640, 2 mM L-glutamine, 25 mM HEPES, 10% heat-inactivated fetal bovine serum (30 min at ...
Understanding how immune cells such as macrophages interact with cancer cells is of increasing interest, as cancer treatments move towards combining both targeted- and immuno- therapies in new treatment regimes. This protocol is using THP-1 cells, a human leukemia monocytic cell line that can be ...
使用PLUS™ 试剂(货号 11514-015)可增强 THP-1 细胞中的转染性能。 我们建议,在络合之前使用 Opti-MEM® I 减血清培养基(货号 31985-062)稀释 DNA 和 Lipofectamine® LTX 试剂。 关于其他专业转染方案(包括关于特定细胞类型的 PLUS™ 试剂和抗...
中文名称:THP-1 Lymphoblastoid cells人单核细胞白血病细胞系英文名称:THP-1 Lymphoblastoid cells 保存条件:液氮保存纯度规格:1x10(6)viable cells/ml 产品类别:实验化学品 "THP-1 Lymphoblastoid cells人单核细胞白血病细胞系 细胞背景资料:该细胞从一名1岁的患有急性单核细胞性白血病的男孩的外周血中分离建立。该细...
The ADCP Reporter Bioassay Effector Cells (THP-1) are provided in thaw-and-use format as cryopreserved cells that can be thawed, plated and used in an assay without the need for cell culture and propagation. When cocultured with a target cell and relevant antibody, the ADCP Reporter Bioassay...
a modified PMA differentiation protocol can enhance macrophage differentiation of THP-1cells and identify increased numbers of mitochondria and lysosomes,resistance to apoptosis and the potency of TLR2responses as important discriminators of the level of macrophage differentiation for transformed cells....
Monocytes are circulatory precursor cells from myeloid origin that can develop into macrophages or dendritic cells upon migration from the blood stream to tissues. Both macrophages and dendritic cells are professional antigen-presenting cells. Monocytes
ChemicalBook provide Chemical industry users with THP-1 cells | THP-1 human monocytic leukemia cell culture medium Boiling point Melting point,THP-1 cells | THP-1 human monocytic leukemia cell culture medium Density MSDS Formula Use,If You also need to T