Understanding how immune cells such as macrophages interact with cancer cells is of increasing interest, as cancer treatments move towards combining both targeted- and immuno- therapies in new treatment regimes. This protocol is using THP-1 cells, a human leukemia monocytic cell line that can be ...
a modified PMA differentiation protocol can enhance macrophage differentiation of THP-1cells and identify increased numbers of mitochondria and lysosomes,resistance to apoptosis and the potency of TLR2responses as important discriminators of the level of macrophage differentiation for transformed cells....
We also present a step-by-step protocol for a transwell co-culture using a human breast cancer cell line, HCC1806, and THP-1-derived macrophages. Finally, we describe the colony formation and migration assays performed on the breast cancer cells after the co-culture ...
中文名称: THP-1 细胞| THP-1 人单核细胞白血病细胞 专用培养基 中文同义词: THP-1 细胞| THP-1 人单核细胞白血病细胞 专用培养基 英文名称: THP-1 cells | THP-1 human monocytic leukemia cell culture medium 英文同义词: THP-1 cells | THP-1 human monocytic leukemia cell culture medium CAS号: ...
In this experiment, the biggest trouble for researchers is how to fix the suspension cells to the glass slide. This protocol describes detailed steps for solving this problem. To ensure the cell viability, it is recommended to use cells that have been in culture between 1 week and 1.5 months...
THP-1 cells are a human monocyte suspension cell line from peripheral blood of a 1 year old infant who had acute mnocytic leukemia. THIS PROTOCOL ACCOMPANIES THE FOLLOWING PUBLICATION https://www.atcc.org/Products/All/TIB-202.aspx DOI dx.doi.org/10.17504/protocols.io.y6gfzbw PROTOCOL CITATIO...
比较不同诱导条件下的细胞THP-1细胞
THP1-Dual™ cells are resistant to the selectable markers blasticidin and Zeocin ®. SAFETY CONSIDERATIONS Biosafety Level 1 HANDLING PROCEDURES Required Cell Culture Medium • Growth Medium: RPMI 1640, 2 mM L-glutamine, 25 mM HEPES, 10% heat-inactivated fetal bovine serum (30 min at ...
THP-1 cells are human monocytic cells that are known to constitutively express most TLRs. In recent years, InvivoGen has engineered THP-1 cell lines as a tool to facilitate answering specific research questions. Examples of engineered THP-1 cell lines, as well as published articles using them...
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