1 Optimized THP-1 differentiation is required for�;Parketal,InflammRes.2007, 56, 45.2 p38α MAP kinase serves cell type-specific inflammatory functions�; Kim et al, Nat Immunol. 2008, 9, 1019.3 PDTC is a potent antioxidant�; Zhu et al, FEBS Letters 2002, 532, 80.4 Anti-Infla...
在Pu•MA系统工作流程中,所有试剂预先装入流式芯片中,然后通过形成免疫复合物的检测通道自动发送。所有清洗步骤都集成到protocol中。protocol完成后,通过读取酶标仪上的吸光度值来进行量化。 抗炎药物 炎症是由带有细胞因子的受体的激活引发的一系列信号事件。激酶激活转录因子上调粘附分子和细胞因子(标志物)。不同的标...
Phorbol 12-myristate 13-acetate (PMA) is commonly used to activate protein kinase C (PKC) and a Standard Reagent for THP1 cell differentiation into macrophages-type cells.
General Protocol of Culturing THP-1 Cells Differentiation of THP-1 and U937 Monocytes into Macrophages Differentiation of THP-1 and U937 Monocytes into Dendritic Cells Controls to Test Viability and Performance of the Model Critical Notes Read-Out of the System ...
(Chanput et al.2014). Furthermore, monocyte-derived macrophages can be polarized into M1, M2a, M2b, and M2c cells. Spencer et al. (2010) published a protocol for THP-1 cell differentiation, showing that THP-1 cells represent a simplified model to study monocyte-macrophage polarization (Qin...
Contamination of this cell line may activate TLRs resulting in differentiation of the monocytes and activation of the reporter gene. • Test Medium: RPMI 1640, 2 mM L-glutamine, 25 mM HEPES, 10% (v/v) heat-inactivated fetal bovine serum (FBS), 100 U/ml penicillin, 100 µg/ml ...
Differentiated THP-1 cells were Figure 5. ERK activity is required for the TCDD-induced TNF-α expression. (A) After differentiation into macrophages with PMA, THP-1 cells were cul- tured in RPMI-1640 containing 0.5% FBS for 24 h before stimulation with TCDD (75 nM). ERK activation was...
Differentiation of THP1 Cells into Macrophages for Transwell Co-culture Assay with Melanoma Cells 来自 NCBI 喜欢 0 阅读量: 161 作者:MP Smith,H Young,A Hurlstone,C Wellbrock 摘要: Understanding how immune cells such as macrophages interact with cancer cells is of increasing interest, as cancer ...
THP-1 cell-line presents a simplified, suitable and reliable model to mimic the monocyte/macrophage polarization, differentiation, functions, possible effects from external stimuli and responses of the surrounding environment. THP-1 is broadly used to learn monocyte/macrophage signalling pathways, mechanis...
These findings suggest a modified PMA differentiation protocol can enhance macrophage differentiation of THP-1 cells and identify increased numbers of mitochondria and lysosomes, resistance to apoptosis and the potency of TLR2 responses as important discriminators of the level of macrophage differentiation ...