1 Optimized THP-1 differentiation is required for�;Parketal,InflammRes.2007, 56, 45.2 p38α MAP kinase serves cell type-specific inflammatory functions�; Kim et al, Nat Immunol. 2008, 9, 1019.3 PDTC is a potent antioxidant�; Zhu et al, FEBS Letters 2002, 532, 80.4 Anti-Infla...
在Pu•MA系统工作流程中,所有试剂预先装入流式芯片中,然后通过形成免疫复合物的检测通道自动发送。所有清洗步骤都集成到protocol中。protocol完成后,通过读取酶标仪上的吸光度值来进行量化。 抗炎药物 炎症是由带有细胞因子的受体的激活引发的一系列信号事件。激酶激活转录因子上调粘附分子和细胞因子(标志物)。不同的标...
比较不同诱导条件下的细胞THP-1细胞
THP-1 cell-line presents a simplified, suitable and reliable model to mimic the monocyte/macrophage polarization, differentiation, functions, possible effects from external stimuli and responses of the surrounding environment. THP-1 is broadly used to learn monocyte/macrophage signalling pathways, mechanis...
(2010) published a protocol for THP-1 cell differentiation, showing that THP-1 cells represent a simplified model to study monocyte-macrophage polarization (Qin 2012). U937 is a pro-monocytic, human myeloid leukaemia cell line and was isolated from the histiocytic lymphoma of a 37 year old ...
These findings suggest a modified PMA differentiation protocol can enhance macrophage differentiation of THP-1 cells and identify increased numbers of mitochondria and lysosomes, resistance to apoptosis and the potency of TLR2 responses as important discriminators of the level of macrophage differentiation ...
THP-1 cells were cultured for 24 h in medium containing 50 ng/mL PMA (Sigma-Aldrich, USA), which was used to induce THP-1 monocyte differentiation into macrophages. Adherent cells were then infected with BCG at an MOI of 10. Construction and transfection of DUSP1 small interfering RNAs ...
Contamination of this cell line may activate TLRs resulting in differentiation of the monocytes and activation of the reporter gene. • Test Medium: RPMI 1640, 2 mM L-glutamine, 25 mM HEPES, 10% (v/v) heat-inactivated fetal bovine serum (FBS), 100 U/ml penicillin, 100 µg/ml ...
Monocytes are the most prominent environment-sensing immune cells in circulation, skilled to shape their microenvironments via cytokine secretion and further differentiation. Both the circulating monocyte subset distribution and the blood plasma EV pattern are characteristic for preeclampsia, a pregnancy ...
Protocol 1 (3.5 mol of PEG/mol of THP) 14.4 mg of THP was dissolved in 10 ml of distillate water; NaCl q.s. to 3.4% was added. The mixing was stirred and then maintained in standing by 2 hours at 2-8° C. The mixing was centrifuged and the precipitate recovered and washed once...