1 Optimized THP-1 differentiation is required for�;Parketal,InflammRes.2007, 56, 45.2 p38α MAP kinase serves cell type-specific inflammatory functions�; Kim et al, Nat Immunol. 2008, 9, 1019.3 PDTC is a potent antioxidant�; Zhu et al, FEBS Letters 2002, 532, 80.4 Anti-Infla...
在Pu•MA系统工作流程中,所有试剂预先装入流式芯片中,然后通过形成免疫复合物的检测通道自动发送。所有清洗步骤都集成到protocol中。protocol完成后,通过读取酶标仪上的吸光度值来进行量化。 抗炎药物 炎症是由带有细胞因子的受体的激活引发的一系列信号事件。激酶激活转录因子上调粘附分子和细胞因子(标志物)。不同的标...
Optional: PMA-induced THP-1 Differentiation Following Phorbol 12-myristate 13-acetate (PMA) treatment, THP1‑Dual™ cells are more sensitive to IFN-inducers, such as transfected Poly(dA:dT), while the response to certain TLR ligands, such as Pam3CSK4, is diminished. Note: PMA treatment ...
(2010) published a protocol for THP-1 cell differentiation, showing that THP-1 cells represent a simplified model to study monocyte-macrophage polarization (Qin 2012). U937 is a pro-monocytic, human myeloid leukaemia cell line and was isolated from the histiocytic lymphoma of a 37 year old ...
General Protocol of Culturing THP-1 Cells Differentiation of THP-1 and U937 Monocytes into Macrophages Differentiation of THP-1 and U937 Monocytes into Dendritic Cells Controls to Test Viability and Performance of the Model Critical Notes Read-Out of the System ...
THP-1 cell line model for tuberculosis: A platform for in vitro macrophage manipulation 6 Conclusion THP-1 cell-line presents a simplified, suitable and reliable model to mimic the monocyte/macrophage polarization, differentiation, functions, possible effects from external stimuli and responses of the...
These findings suggest a modified PMA differentiation protocol can enhance macrophage differentiation of THP-1cells and identify increased numbers of mitochondria and lysosomes,resistance to apoptosis and the potency of TLR2responses as important discriminators of the level of macrophage differentiation for ...
Contamination of this cell line may activate PRRs, such as TLRs, resulting in differentiation of the monocytes and activation of PRR signaling pathways. • Test Medium: RPMI 1640, 2 mM L-glutamine, 25 mM HEPES, 10% (v/v) heat-inactivated fetal bovine serum (FBS), 100 U/ml penicillin,...
比较不同诱导条件下的细胞THP-1细胞
THP-1 cells were cultured for 24 h in medium containing 50 ng/mL PMA (Sigma-Aldrich, USA), which was used to induce THP-1 monocyte differentiation into macrophages. Adherent cells were then infected with BCG at an MOI of 10. Construction and transfection of DUSP1 small interfering RNAs ...