This research optimized the induction method on THP-1 cell differentiation from three aspects and delineated the transcriptomic profile of PMA-induced THP-1 cells, laying a foundation for the construction method of cell model and for the functional study of macrophage....
This protocol is using THP-1 cells, a human leukemia monocytic cell line that can be differentiated into macrophages. This allows studying the effects of the macrophage secretome on cancer cells (on e.g. growth, drug response or gene expression) in co-cultures without direct cell contact ...
D3(VD 3)are stimuli commonly used to induce macrophage differentiation in monocytic cell lines but the extent of differentiation in comparison to primary tissue macrophages is unclear.We have compared the phenotype of the promonocytic THP-1cell line after various protocols of differentiation utilising...
不过要注意,后续使用的培养基里不能含有PMA哦。至于M1和M2的实验,我暂时还没经验,但可以参考一些文献,比如《Optimization of differentiation and transcriptomic profile of THP-1 cells into macrophage by PMA》和《Standardized protocols for differentiation of THP-1 cells to macrophages with distinct M(IFN-γ ...
Ding Q,Jin T,Wang Z,Chen Y.Catalase potentiates retinoic acid- induced THP-1 monocyte differentiation into macrophage through inhi- bition of peroxisome proliferator-activated receptor. Journal of Leukocyte Biology . 2007Ding Q,Jin T,Wang Z,et al. Catalase potentiates retinoic acid- induced THP-...
比较不同诱导条件下的细胞THP-1细胞
of macrophage cells during differentiation and immune activation.Nat Commun. 2022 Oct 4;13(1):...
their differentiation in the monocyte/macrophage pathway. Aimof the present study is the investigation of the in vitro interaction of two synthetic injectable hydrogels, polyalkylimide (PAI) and polyvinyl al- cohol (PVA), with U937 and THP-1 cells. Meas- ...
After THP-1 macrophage differentiation, we transfected 50 nM of hsa-miR-372-3p, hsa-miR-373-3p, and/or hsa-miR-520d-3p mimics or inhibitors or the positive and negative controls (miRVana, Thermo Scientific), previously incubated with the HiPerfect Transfection Reagent (Qiagen). Infection was...
Theanti-atherogeniceffectsofeicosapentaenoic anddocosahexaenoicacidaredependentonthe stageofTHP-1macrophagedifferentiation SheilaMcCartan a,b ,LesleyA.Powell a ,BrianD.Green b ,JaneMcEneny a , AnnMcGinty a, * a CentreforPublicHealth,SchoolofMedicine,Dentistry&BiomedicalSciences,Belfast,NorthernIreland,UK ...