[3] Sedlyarov V, Eichner R, Girardi E, et al. The bicarbonate transporter SLC4A7 plays a key role in macrophage phagosome acidification[J]. Cell host & microbe, 2018, 23(6): 766-774. e5. [4] Benyoucef A, Marchitto L, Touzot F. CRISPR gene-engineered CYBBko THP-1 cell lines ...
[3] Sedlyarov V, Eichner R, Girardi E, et al. The bicarbonate transporter SLC4A7 plays a key role in macrophage phagosome acidification[J]. Cell host & microbe, 2018, 23(6): 766-774. e5. [4] Benyoucef A, Marchitto L, Touzot F. CRISPR gene-engineered CYBBko THP-1 cell lines ...
[1]Chanput W, Mes JJ, Wichers HJ. THP-1 cell line: an in vitro cell model for immune modulation approach. Int Immunopharmacol. 2014;23(1):37-45. [2]Jaramillo-Valverde L, Levano KS, Capristano S, et al. CXCR4 Knockdown Via CRISPR/CAS9 in a Tumor-Associated Macrophage Model Decreases...
[3] Sedlyarov V, Eichner R, Girardi E, et al. The bicarbonate transporter SLC4A7 plays a key role in macrophage phagosome acidification[J]. Cell host & microbe, 2018, 23(6): 766-774. e5. [4] Benyoucef A, Marchitto L, Touzo...
015. PMID: 15530839.[2].Chanput W, Mes JJ, Wichers HJ. THP-1 cell line: an in vitro cell model for immune modulation approach. Int Immunopharmacol. 2014;23(1):37-45. [3].Tominaga T, Suzuki M, Saeki H, Matsuno S, Tachibana T, Kudo T. Establishment of an activated macrophage ...
[3] Sedlyarov V, Eichner R, Girardi E, et al. The bicarbonate transporter SLC4A7 plays a key role in macrophage phagosome acidification[J]. Cell host & microbe, 2018, 23(6): 766-774. e5. [4] Benyoucef A, Marchitto L, Touzot F. CRISPR gene-engineered CYBBko THP-1 cell lines ...
THP-1;巨噬细胞;佛波酯;流式细胞术巨噬细胞(macrophage,Mφ)是一类由血液中单核细胞分化而来的固有免疫细胞,在机体免疫反应中发挥重要作用[1]。但是由于细胞数量少,分离过程较繁琐[2,3],因此多用佛波酯(phorbol 12-myristate 13-acetate, PMA)诱导THP-1单核细胞系分化而成的THP-1-Mφ作为研究巨噬细胞功能...
the expression of TNF-α and IL-1β wasdetected by real-time fluorescence quantitative polymerase chain reaction (qRT-PCR), and the effect of M1macrophage co-cultured with esophageal squamous cell carcinoma cell line Eca109 on apoptosis, proliferationand migration of Eca109 cells were detected by...
| 2.2 Monocyte Derived Macrophage单核细胞的诱导分化 △点击放大图片 实验步骤 1 2 1 实验流程 ①根据细胞生长状态和形态取2~4代细胞进行实验。收集形态典型、生长状态良好的THP-1进行实验[THP-1细胞呈单个圆形悬浮,密度约(5~10)×106/mL,分布均匀,折光度较好]。 ②THP-1细胞使用含有10%胎牛血清、1%谷...
Macrophage-independent oxidation of LDL, mediated by the addition of copper ions, was inhibited by cystine and cysteine in phosphate buffered saline, but not in Hams F10 medium. The glutathione content of THP-1 macrophages was also dependent upon the presence of cysteine or cystine in the medium...