可适当增加β-巯基乙醇的比例（不超过标准量的1.5倍）。参考文献 [1].Mantovani A, Sica A, Sozzani S, Allavena P, Vecchi A, Locati M. The chemokine system in diverse forms of macrophage activation and polarization. Trends Immunol. 2004 Dec;25(12):677-86. doi: 10.1016/j.it.2004.09.015...
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T. marneffei produces a number of different laccases linked to fungal virulence. To understand the role of the various laccases in T. marneffei, laccase-encoding genes were investigated. Targeted single, double and triple gene deletions of laccases encoding lacA, lacB, and lacC showed no ...
Macrophage Virulence Assays 一、THP-1细胞的制备 1、第一天,加5x106THP-1于50ml完全培养基1640,于225cm2培养瓶中培养(根据实验需要,设置培养体积)。 2、于CO2培养箱中直立培养3d。 3、第四天,将培养瓶放倒,继续培养2d。 4、第六天,另外加入50ml新鲜完全培养基。 5、继续培养2d。 6、第八天,轻轻震荡培养...
10. DENG L S, JIAN Z J, XU T, et al. Macrophage polarization:an important candidate regulator for lung diseases[J]. Molecules, 2023,28(5):2379. 11. KROEMER G. Mitochondrial control of apoptosis:an introduction[...
PMA诱导THP-1步骤：将处于对数生长期的THP-1细胞离心，用RPMI-1640培养基重悬；加入PMA，显微镜下细胞...
方法 以 160 nmol/ L 的佛 经 油红 0 染色 E stablishm ent a nd Identification fl0r M acro phag e —der ived F o 锄WANG Shuling, PAN Huaxin, WANG Peixun(Guangzh0u university of Chinese medieine, Guangzh0u 5 1 0405 , China) Abstract : o bl jective To establish a macrophage — ...
THP-1;巨噬细胞;佛波酯;流式细胞术巨噬细胞(macrophage,Mφ)是一类由血液中单核细胞分化而来的固有免疫细胞,在机体免疫反应中发挥重要作用[1]。但是由于细胞数量少,分离过程较繁琐[2,3],因此多用佛波酯(phorbol 12-myristate 13-acetate, PMA)诱导THP-1单核细胞系分化而成的THP-1-Mφ作为研究巨噬细胞功能...
图片引自用:Shi J, Li Q, Sheng M, et al. The Role of TLR4 in M1 Macrophage-Induced Epithelial-Mesenchymal Transition of Peritoneal Mesothelial Cells. Cell Physiol Biochem. 2016;40(6):1538–1548. 本文来自流式中文网(flowcyto.cn)欢迎转发到朋友圈,但谢绝复制粘贴转载如有需要请联系mail@flowcyto....
[2]Jaramillo-Valverde L, Levano KS, Capristano S, et al. CXCR4 Knockdown Via CRISPR/CAS9 in a Tumor-Associated Macrophage Model Decreases Human Breast Cancer Cell Migration. Cureus. 2021;13(12):e20842. 赛业生物Smart-CRISPR™基因编辑系统 ...