可适当增加β-巯基乙醇的比例（不超过标准量的1.5倍）。参考文献 [1].Mantovani A, Sica A, Sozzani S, Allavena P, Vecchi A, Locati M. The chemokine system in diverse forms of macrophage activation and polarization. Trends Immunol. 2004 Dec;25(12):677-86. doi: 10.1016/j.it.2004.09.015...
The results showed that luteolin exhibited a potent preventive effect on THP-1 macrophage pyroptosis, as evidenced by the increase in cell viability and the decrease in LDH release. Moreover, luteolin was found to significantly reduce the expression of NLRP3, pro-CASP-1 and CASP-1, which ...
Key Words: macrophage atherosclerosis cholesterol triglyceride cyclodextrin F oam cell macrophages are involved in all stages of atherosclerosis. 1 Unregulated uptake of modified LDL, such as acetylated LDL (AcLDL), via the scavenger receptor (ScR), is considered important in the accumulation of unes-...
Int Immunopharmacol. 2014;23(1):37-45. [3].Tominaga T, Suzuki M, Saeki H, Matsuno S, Tachibana T, Kudo T. Establishment of an activated macrophage cell line, A-THP-1, and its properties. Tohoku J Exp Med. 1998 Oct;186(2):99-119.doi: 10.1620/tjem.186.99. PMID: 10223614....
5. F Kianoush, M Nematollahi, J D Waterfield, etc. Regulation of RAW264.7 macrophage ...
[2]Jaramillo-Valverde L, Levano KS, Capristano S, et al. CXCR4 Knockdown Via CRISPR/CAS9 in a Tumor-Associated Macrophage Model Decreases Human Breast Cancer Cell Migration. Cureus. 2021;13(12):e20842. 赛业生物Smart-CRISPR™基因编辑系统 ...
Macrophage Virulence Assays 一、THP-1细胞的制备 1、第一天,加5x106THP-1于50ml完全培养基1640,于225cm2培养瓶中培养(根据实验需要,设置培养体积)。 2、于CO2培养箱中直立培养3d。 3、第四天,将培养瓶放倒,继续培养2d。 4、第六天,另外加入50ml新鲜完全培养基。 5、继续培养2d。 6、第八天,轻轻震荡培养...
Cell surface proteoglycans modulate net synthesis and secretion of macrophage apolipoprotein E. J. Biol. Chem. 271: 13454-13460, 1996. PubMed: 8662812 Sando GN, et al. Induction of ceramide glucosyltransferase activity in cultured human keratinocytes. J. Biol. Chem. 271: 22044-22051, 1996. Pub...
[3] Sedlyarov V, Eichner R, Girardi E, et al. The bicarbonate transporter SLC4A7 plays a key role in macrophage phagosome acidification[J]. Cell host & microbe, 2018, 23(6): 766-774. e5. [4] Benyoucef A, Marchitto L, Touzo...
[3] Sedlyarov V, Eichner R, Girardi E, et al. The bicarbonate transporter SLC4A7 plays a key role in macrophage phagosome acidification[J]. Cell host & microbe, 2018, 23(6): 766-774. e5. [4] Benyoucef A, Marchitto L, Touzot F. CRISPR gene-engineered CYBBko THP-1 cell lines ...