细胞学堂｜RAW264.7和THP-1细胞 单核细胞（Monocytes）来源于骨髓中的造血干细胞，是血液中最大的血细胞，同时也是免疫细胞，参与机体的免疫防御。巨噬细胞（Macrophages）是一种位于组织内的白血球，由血液中的单核细胞穿出血管、进入组织后分化形成，体积增大，溶酶体增多，吞噬功能增强。单核细胞和巨噬细胞同属于...
单核细胞(Monocytes)来源于骨髓中的造血干细胞,是血液中最大的血细胞,同时也是免疫细胞,参与机体的免疫防御。巨噬细胞(Macrophages)是一种位于组织内的白血球,由血液中的单核细胞穿出血管、进入组织后分化形成,体积增大,溶酶体增多,吞噬功能增强。单核细胞和巨噬细胞同属于吞噬细胞,是机体重要的免疫细胞,能够吞噬并...
参考文献: [1] Long H, Lin H, Zheng P, et al. WTAP mediates the anti-inflammatory effect of Astragalus mongholicus polysaccharide on THP-1 macrophages[J]. Frontiers in Pharmacology, 2022, 13: 1023878. [2] Horiguchi Y, Ohta N, Yamamoto S, et al. Midazolam suppresses the lipopolysaccharide...
基于THP-1细胞的众多优点,不少科研人员以THP-1细胞为遗传背景进行基因修饰,再分化巨噬细胞,开展免疫和炎症等领域的研究。比如下面的报道[2],作者通过CRISPR-Cas9系统在THP-1细胞中敲除CXCR4,再分化为肿瘤相关巨噬细胞(tumor-associated macrophages,TAMs),从而研究在TAMs中敲除CXCR4对乳腺癌细胞迁移的影响。 作者将T...
Significance: Are PMA-Differentiated THP-1 Cells a Reliable Substitute for Blood-Derived Macrophages ...
The integration of THP-1 macrophages was optimized and their effect on the endothelial lumen was characterized, focusing on permeability and structural integrity. The device was then employed to examine the influence of macrophages on neutrophil response to infection with the bacterial pathogen ...
巨噬细胞(Macrophages)是一种位于组织内的白血球,由血液中的单核细胞穿出血管、进入组织后分化形成,体积增大,溶酶体增多,吞噬功能增强。单核细胞和巨噬细胞同属于吞噬细胞,是机体重要的免疫细胞,能够吞噬并杀伤多种入侵的病原微生物,同时激活淋巴细胞或其他免疫细胞,激活机体的免疫响应。单核-巨噬细胞是研究细胞生物...
M1 and M2 macrophages derived from THP-1 cells differentially modulate the response of cancer cells to etoposide 热度: ox-LDL通过PKA_PKC途径上调PHF2促进THP-1巨噬细胞炎症反应 热度: 去甲基化药物联合去乙酰化酶抑制剂对THP-1细胞中PTPN6表达的作用 ...
[1]Long H, Lin H, Zheng P, et al. WTAP mediates the anti-inflammatory effect of Astragalus mongholicus polysaccharide on THP-1 macrophages[J].Frontiers in Pharmacology, 2022, 13: 1023878. [2]Horiguchi Y, Ohta N, Yamamoto S, et al. Midazolam suppresses the lipopolysaccharide-stimulated immun...
HLA-DR were detected by flow cytometry. Results The macrophagesstimulated by PMA became adherent;THP-1-M1 and THP-1-M2 lost their spherical morphology,appeared more irregularwith many obvious projections. The expression of CD83,CD1a,CD11c,HLA-DR which had the function of antigenpresenting on the...