which can be differentiated into macrophages by PMA, is widely used in researches on pathogen infection and host innate immunity, but reports on the induction methods of PMA are different and lack a unified standard, and the transcriptome characteristics of macrophage c...
Antoine Fattaccioli, etc. M1 and M2 macrophages derived from THP-1 cells differentially modulate th...
Understanding how immune cells such as macrophages interact with cancer cells is of increasing interest, as cancer treatments move towards combining both targeted- and immuno- therapies in new treatment regimes. This protocol is using THP-1 cells, a human leukemia monocytic cell line that can be ...
将处于对数生长期的THP-1细胞离心，用RPMI-1640培养基重悬；加入PMA，显微镜下细胞计数，调整THP-1细胞...
与THP-1-Mφ比较,* P 0.05。图2 CD14和CD68分子在不同巨噬细胞上表达的差异Note: “……” Isotype control; “-”CD14 and CD68;A:The expression of CD molecules were detected by flow cytometry;B:The differences of CD molecules on different cells were analyzed by histogram.Compared with the ...
thp1细胞 细胞名称: 人单核细胞白血*(STR鉴定正确) thp1细胞细胞别称: THP1; THP 1; THPI; THP-1(ATCC); O-THP-1; Tohoku Hospital Pediatrics-1 货号:TCH-C361 规格:1×10⁶cells/T25 培养瓶 价格:1500元 (干冰运输需另加200元干冰费) ...
which the CD8+ T cells response is strongly weaken [6]. Although the concept of TAMs has been proposed for decades, the mechanisms by which the tumor polarizes macrophages to 35 anti-inflammatory and immune suppressive phenotypes are still poorly understood. In this study, ...
TCDD increases mRNA and protein expression of TNF-α in PMA-differentiated THP-1 cells. (A) THP-1 cells were differentiated into macro- phages by culturing them with 150 nM PMA for 2 days. Various concentrations of TCDD (12.5-100 nM) were added to THP-1 macrophages for 24 h, and TNF...
结论耵口一1来源巨噬细胞的诱导分化及CDl37/C Dl37L逆向信号在其中的作用 结论 1.PMA刺激THP一1成熟为巨噬细胞的过程中,LP S及IFN-Y可使iNOS、IL一6及TNF— Q上调,诱导其向M1极化,IL一4可使iNOS、IL 2 一6及TNF-Q显著下调并升高IL一10的表达,诱导 巨噬细胞向M2极化,为今后体内功能实验打下基础。
A E, RE N A, et al. Standardized protocols for differentiation of THP-1 cells to macrophages ...