The human monocytic leukaemia cell line THP-1, when stimulated with phorbol ester, shares many properties with human monocyte-derived macrophages. Oxidation of LDL by these cells was characterised by depletion of 伪-tocopherol, increases in thiobarbituric acid reactive substances and increases in ...
THP1-Dual™ hTLR3 cells derive from THP-1 monocytes and overexpress the human TLR3 gene as well as all elements important in its signaling cascade. They also feature two inducible reporter genes, allowing the concomitant study of the IRF and NF-κB pat
TLR7 is mainly found in plasmacytoid dendritic cells (pDCs) and B cells, whereas TLR8 is highly expressed in monocytes, monocyte-derived DCs (mDCs), and macrophages [3]. TLR7 and TLR8 trafficking and signaling Upon viral infection, the chaperone protein UNC93B1 interacts with TLR7 and ...
Collectively, 1m increases ABCA1 expression in human THP-1 macrophages. The mechanisms involve the activation of the LXRα-ABCA1 pathway and suppression of certain microRNAs that regulate ABCA1 expression. 展开 关键词: atherosclerosis, cholesterol efflux, ATP-binding cassette transporters A1, chalcone ...
Figure 1 (A) Schematic representation of unstimulated (M0 controls) and pro-inflammatory (M1) THP-1-derived macrophages; (B) Typical 1H NMR spectrum obtained for polar extracts of THP-1-derived macrophages; (C) Multivariate analysis of spectral data from M0 and M1 macrophages; D) Heatmap of...
Here we report the pro-inflammatory effect of ImI, a well characterized conotoxin that inhibits α7 nAChRs, on differentiated THP-1 pre-monocyte macrophages (MDM) obtained by phorbol 12-myristate 13 acetate (PMA) treatment. Enzyme-linked immunosorbent assay (ELISA) performed on supernatant fluids ...
In HIV-1Bal-infected macrophages, Lipofectamine (up to 8 μM) and Lipofectin (up to 40 μM) were not toxic after a 4-h treatment, while DMRIE reagent at 40 μM was toxic. While a 4-h treatment of THP-1 /HIV-1 IIIB cells with the cationic liposomes was not toxic, even up to ...
We have recently profiled the 3D organization and long-range interactome of human THP-1 monocytes and THP-1-derived macrophages through deeply sequenced in situ Hi-C experiments. High-resolution mapping of DNA loops identified both static and dynamic loop-based regulation of key macrophage genes du...
1.Expression of genes for inflammatory cytokines in human monocytic cell line THP-1 during exposure to isoflurane;目的研究异氟醚对人类单核细胞株(THP-1)细胞、白细胞介素-1β(IL-1β)、IL-8、肿瘤坏死因子-α(TNF-α)、干扰素-γ(IFN-γ)、IL-4和IL-10mRNA表达的影响。 3)monocyte line Raw264.7...
In addition they have a high absorption capacity for latex micro beads and express a cytokine profile that resembles macrophages. Ganoderma lucidum polysaccharides have been found to induce monocytic THP-1 cells into dendritic cells [11] if added together with GM-CSF/IL-4, and recently it was ...