The results showed that luteolin exhibited a potent preventive effect on THP-1 macrophage pyroptosis, as evidenced by the increase in cell viability and the decrease in LDH release. Moreover, luteolin was found to significantly reduce the expression of NLRP3, pro-CASP-1 and CASP-1, which ...
[1].Mantovani A, Sica A, Sozzani S, Allavena P, Vecchi A, Locati M. The chemokine system in diverse forms of macrophage activation and polarization. Trends Immunol. 2004 Dec;25(12):677-86. doi: 10.1016/j.it.2004.09.015. PMID: 15530839.[2].Chanput W, Mes JJ, Wichers HJ. THP...
[1]Chanput W, Mes JJ, Wichers HJ. THP-1 cell line: an in vitro cell model for immune modulation approach. Int Immunopharmacol. 2014;23(1):37-45. [2]Jaramillo-Valverde L, Levano KS, Capristano S, et al. CXCR4 Knockdown Via CRISPR/CAS9 in a Tumor-Associated Macrophage Model Decreases...
将THP-1细胞经PMA诱导为巨噬细胞后，可用RPMI-1640培养基配制氧化修饰低密度脂蛋白OX-LDL，使其终浓度...
Inhibition of macrophage scavenger receptor activity by tumor necrosis factor-alpha is transcriptionally and post-transcriptionally regulated. J. Biol. Chem. 271: 7767-7773, 1996. PubMed: 8631819 Lucas M, Mazzone T. Cell surface proteoglycans modulate net synthesis and secretion of macrophage ...
(C)图是用Real-time PCR进一步证实M1 细胞中iNOs、TNF-α和 CXCL10 的 mRNA 水平显著上调,而 M2 细胞中 Arg-1 和 CD163 的 mRNA 水平显著升高。 图片引自用:Shi J, Li Q, Sheng M, et al. The Role of TLR4 in M1 Macrophage-Induced Epithelial-Mesenchymal Transition of Peritoneal Mesothelial Cells...
Key Words: macrophage atherosclerosis cholesterol triglyceride cyclodextrin F oam cell macrophages are involved in all stages of atherosclerosis. 1 Unregulated uptake of modified LDL, such as acetylated LDL (AcLDL), via the scavenger receptor (ScR), is considered important in the accumulation of unes-...
至于M1和M2的实验,我暂时还没经验,但可以参考一些文献,比如《Optimization of differentiation and transcriptomic profile of THP-1 cells into macrophage by PMA》和《Standardized protocols for differentiation of THP-1 cells to macrophages with distinct M(IFN-γ + LPS), M(IL-4) and M(IL-10) ...
一、THP-1细胞的极化 1) THP-1细胞铺板,将佛波酯PMA(100 ng/ml)加入完全培养基中,诱导其向巨噬细胞的成熟; 2) 48小时后THP-1细胞成熟为巨噬细胞,外观圆形高折光,从悬浮状态快速变成贴壁状态; 3) 将成熟的巨噬细胞消化下来(Accutase酶消化:专门用于消化贴壁细胞),重新铺板,诱导向M1-like、M2-like的极化。
THP-1细胞系是从一名患有急性单核细胞白血病的1岁小男孩的外周血中分离得到的,自1980年建系以来,THP-1细胞被广泛用于单核细胞和巨噬细胞相关的机制、信号通路以及营养和药物运输等研究中。相对于U937、HL-60、ML-2等白血病细胞系,THP-1更有类似人原代单核细胞的形态和功能特征(包括细胞分化标记)。相对于人外周...