THP-1细胞系是从一名患有急性单核细胞白血病的1岁小男孩的外周血中分离得到的[1],自1980年建系以来,THP-1细胞被广泛用于单核细胞和巨噬细胞相关的机制、信号通路以及营养和药物运输等研究中。相对于U937、HL-60、ML-2等白血病细胞系,THP-1更有类似人原代单核细胞的形态和功能特征(包括细胞分化标记)。相对于人...
有研究者利用CRISPR/Cas9技术,在THP-1细胞中进行CYBB基因的敲除和点突变c.90C>G(该点突变曾在一例CGD患者中被发现),成功构建了首个CGD的细胞模型。对比野生型的THP-1细胞,研究者构建的2个KO克隆(#3和#27)和1个点突变克隆(#2,c.90C>G)在经过PMA和LPS诱导后均出现了H2O2水平降低,同时IL-1β、TNF-α和I...
有研究者利用 CRISPR/Cas9 技术,在 THP-1 细胞中进行 CYBB 基因的敲除和点突变 c.90C > G(该点突变曾在一例 CGD 患者中被发现),成功构建了首个 CGD 的细胞模型。 对比野生型的 THP-1 细胞,CGD 的细胞模型在经过 PMA 和 LPS 诱导后均出现了 H...
[1].Mantovani A, Sica A, Sozzani S, Allavena P, Vecchi A, Locati M. The chemokine system in diverse forms of macrophage activation and polarization. Trends Immunol. 2004 Dec;25(12):677-86. doi: 10.1016/j.it.2004.09.015. PMID: 15530839.[2].Chanput W, Mes JJ, Wichers HJ. THP...
the expression of TNF-α and IL-1β wasdetected by real-time fluorescence quantitative polymerase chain reaction (qRT-PCR), and the effect of M1macrophage co-cultured with esophageal squamous cell carcinoma cell line Eca109 on apoptosis, proliferationand migration of Eca109 cells were detected by...
[1]Chanput W, Mes JJ, Wichers HJ. THP-1 cell line: an in vitro cell model for immune modulation approach. Int Immunopharmacol. 2014;23(1):37-45. [2]Jaramillo-Valverde L, Levano KS, Capristano S, et al. CXCR4 Knockdown Via CRISPR/CAS9 in a Tumor-Associated Macrophage Model Decreases...
《THP.1 cel l was detected wi th a fl ow eytometry,and cel l mortal i tv ratewas detected bytrypan bl ue stai ni ng method.R酷l Il ts Adenovi ms exhi bi tedhi ghtⅢsfecti onemci encyofTHP—lmacrophage,andl ow cel l ul ardamage.But itmayhavestrongefkcts on cel l ul ar ...
andRAWmacrophagecells.Whereasthenonhumancelllinesallesterifiedover30%of2-hydroxypropyl- -cyclodextrin(hp- -CD)–deliveredcholesterolwithin6hours,THP-1cellsesterified 8.0%ofincorporatedcholesterol.KineticsofcholesteroleffluxfromAcLDL-loadedTHP-1cellswerefirstinvestigatedafterloadingwithonlyFC,andinteractionsbetweenefflux...
[3] Sedlyarov V, Eichner R, Girardi E, et al. The bicarbonate transporter SLC4A7 plays a key role in macrophage phagosome acidification[J]. Cell host & microbe, 2018, 23(6): 766-774. e5. [4] Benyoucef A, Marchitto L, Touzot F. CRISPR gene-engineered CYBBko THP-1 cell lines ...
结论单独使用PMA刺激THP-1细胞所分化而来的巨噬细胞抗原提呈能力较弱,基本处于静息状态。 THP-1;巨噬细胞;佛波酯;流式细胞术巨噬细胞(macrophage,Mφ)是一类由血液中单核细胞分化而来的固有免疫细胞,在机体免疫反应中发挥重要作用[1]。但是由于细胞数量少,分离过程较繁琐[2,3],因此多用佛波酯(phorbol 12-...