THP-1 macrophage2022 Elsevier B.V.. All rights reserved.Importance of the work: Cold-pressed rice bran oil has been widely consumed, however its pathway involving anti-inflammatory activity has not been elucidated.Objectives: To investigate the potential of CPRBO emulsion as a delivery system and...
OPEN Citation: Cell Death and Disease (2016) 7, e2527; doi:10.1038/cddis.2016.433 Official journal of the Cell Death Differentiation Association www.nature.com/cddis Hypericin-mediated sonodynamic therapy induces autophagy and decreases lipids in THP-1 macrophage by promoting ROS-dependent nuclear ...
The pretreatment of cultures with NAC and NaN3 not only significantly reduced ROS generation but also rescued the SDT-induced macrophage cytotoxicity (Figure 4A–C). Moreover, to confirm the relationship between ROS and a decreased ΔΨm, ΔΨm was measured in cells pretreat...
of macrophage following HY-SDT with or without ABCA1 knockdown via ABCA1 siRNA. We found ABCA1 siRNA #2 decreased the protein level of ABCA1 (Supplementary Figure S6a). Moreover, the lipid efflux promoted following HY-SDT was reversed by ABCA1 siRNA (Figure 6e). These results suggest that...
THP-1 cells display a monocytic-like cell line with distinct monocytic markers [55]. Treatment with phorbol esters leads to differentiation to M0 macrophages, behaving similarly to native macrophages and thus providing a suitable model for studies of macrophage responses [56]. Their differentiation le...
In THP-1 cells,HO-1 induction counteracted the effect of TNF-induced cell death via Nrf2 activation [18].This is potentially of importance to myco-bacterial infection as it appears that macrophage apoptosis con-tributes to host defense [19].The role of CO in mycobacterial in-fection has ...
their differentiation in the monocyte/macrophage pathway. Aimof the present study is the investigation of the in vitro interaction of two synthetic injectable hydrogels, polyalkylimide (PAI) and polyvinyl al- cohol (PVA), with U937 and THP-1 cells. Meas- ...
For Rap1 Activation Assay, THP-1 monocytes were seeded on 6-well plates at a concentration of 1 × 106 per well and differentiated into macrophage-like cells as described above. Then, cells were treated with PWO577 or SY007 (100 μM) or DMSO for 1, 2, 5, 10 and 30 min, washed ...
Fluorescent competitive assays involving ImI, α-bungarotoxin and nicotine using MDM and the murine macrophage RAW 264.7 suggest a common binding site in the α7 receptor. This work extends the application of conotoxins as molecular probes to non-excitatory cells, such as macrophages and supports ...
R. et al. Macrophage expression of peroxisome proliferator-activated receptor-alpha reduces atherosclerosis in low- density lipoprotein receptor-deficient mice. Circulation. 116, 1404–1412 (2007). 30. Shen, W. et al. Negatively regulating TLR4/NF-κB signaling via PPARαin endotoxin-...