A flow cytometry‐based erythrophagocytosis assay was established using phorbol 12‐myristate 13‐acetate (PMA)‐treated THP‐1 cells and RBCs labelled with PKH26 to assess allo‐ and autoantibodies to RBCs. Materials and Methods THP‐1 cells were differentiated into macrophage‐like cells by ...
We used our previously established FACS-based phagocytosis assay20 and performed a genome-wide CRISPR screen in THP-1 cells phagocytosing S. aureus particles. Subsequently, the selected hits were validated in single gene knockout experiments and characterized with additional phagocytosis target ...
流式细胞术检测巨噬细胞CD86和CD206的表达情况;酶联免疫吸附试验(enzyme-linked immunosorbent assay,ELISA)检测细胞上清液白细胞介素(interleukin,IL)-6、IL-10、肿瘤坏死因子(tumor necrosis factor,TNF)-α和肿瘤生长因子(tumor growth factor,TGF)-β 4种细胞因子的分泌水平;酶法检测细胞内甘油三酯(triglyceride,...
sometimes a mimic of digestion is applied to the samples prior to analysis. It is highly recommended to determine the toxicity of such solvents or digestion reagents, as well as their effect on the read-out parameters to be used for the cell assay, in a titration that at ...
is another classic agent used to induce differentiation of THP-1 into a macrophage-like phenotype. However, both of these chemicals regulate differentsignalling pathways, and treatment with PMA has advantages over 1α, 25(OH)2D3in developing a more mature phenotype with a higherphagocytosisrate, ...
Moreover, a decrease in neutrophilic phagocytosis has been observed in patients with uncontrolled diabetes, and peripheral blood monocytes from patients with diabetes with periodontal disease, secrete significantly more prostaglandin E2 (PGE2) [6, 8]. Increased inflammatory responses to infection and a ...
However, Mtb can survive inside macrophages post-phagocytosis using various mechanisms, including inhibition of apoptosis and oxidative stress5. Apoptotic pathways can be classified as endogenous, exogenous, and endoplasmic reticulum pathways6. Regardless of the pathway, cells undergoing apoptosis eventually ...
38. Graham JE, Clark-Curtiss JE: Identification of Mycobacterium tuberculosis RNAs synthesized in response to phagocytosis by human macrophages by selective capture of transcribed sequences (SCOTS). Proc Natl Acad Sci USA 1999, 96:11554–11559. 39. Domenech P, Honoré N, Heym B, Cole ST: ...
mlfor24h,andtheactivatedgiantmacrophagehadthephagocytosisforchickenredbloodcells. Conclusion LPScouldactivatetheproliferationofmacrophagesandincreasethephagoeytosis. Keywords: LPS; THP一1cells; optimizingfactors THP.1细胞株来源于急性单核细胞白血病人,
While TCDD treatment does not alter macrophge-mediated antigen pre- sentation, phagocytosis or tumor cell cytolysis, there is evidence suggesting that TCDD stimulates production of inflammatory mediators such as reactive oxygen species and inflammatory cyto- kines (Clark et al., 1991; Alsharif et ...