将THP-1细胞诱导为M0→M1→M2的一些关键细节 图片引用自:https://www.caltagmedsystems.co.uk/information/pma-standard-reagent-for-thp1-cell-differentiation/ 为了成功地将THP-1细胞诱导成M0巨噬细胞,并随后将其极化为M1或M2巨噬细胞,需要考虑以下几个关键因素和方法: THP-1细胞向M0巨噬细胞的分化 1.PMA诱...
1. Optimized THP-1 differentiation is required for...; Park et al, Inflamm Res. 2007, 56, 45. 2. p38α MAP kinase serves cell type-specific inflammatory functions ...; Kim et al, Nat Immunol 2008, 9, 1019. 3. PDTC is a potent antioxidant...; Zhu et al, FEBS Letters 2002,...
This research optimized the induction method on THP-1 cell differentiation from three aspects and delineated the transcriptomic profile of PMA-induced THP-1 cells, laying a foundation for the construction method of cell model and for the functional study of macrophage....
Phorbol 12-myristate 13-acetate (PMA) is commonly used to activate protein kinase C (PKC) and a Standard Reagent for THP1 cell differentiation into macrophages-type cells.
图片引用自：https://www.caltagmedsystems.co.uk/information/pma-standard-reagent-for-thp1-cell-differentiation/ 在实际实验过程中，THP-1的状态是影响诱导效果的最大因素。此外，由于诱导时间较长，随时关注诱导过程中的细胞状态，也十分关键。针对这一难点，富衡推出THP-1成巨噬细胞诱导试剂盒，试剂盒中不仅包括...
图片引用自:https://www.caltagmedsystems.co.uk/information/pma-standard-reagent-for-thp1-cell-differentiation/ 在实际实验过程中,THP-1的状态是影响诱导效果的最大因素。此外,由于诱导时间较长,随时关注诱导过程中的细胞状态,也十分关键。针对这一难点,富衡推出THP-1成巨噬细胞诱导试剂盒,试剂盒中不仅包括各阶...
This is an important aspect as it removes the presence of any phagocytic aspect to changes in the cancer cell number and behaviour. The in vitro THP-1 monocyte differentiation into polarized macrophages was used to study the effects of both M1 and M2 type populations of macrophages on melanoma...
urement of differentiation into macrophages will be analyzed by means of biochemical and mor- phological assays. MATERIALS AND METHODS Cell culture - THP-1 cells, leukemic cell line de- rived from a patient with acute monocytic leukae-
• 环境控制模块系统通过CellReporterXpress™ 成像和分析软件进行控制SpectraMax® iD5 多功能酶标仪是用来测量吸光度，计算浓度和 EC50 值。参考文献 1 Optimized THP-1 differentiation is required for�;Parketal,InflammRes.2007, 56, 45.2 p38α MAP kinase serves cell type-specific inflammatory ...
Carmen Feinweber, Sonja Luckhardt, etc. THP-1 and human peripheral blood mononuclear cell-derived ...