将THP-1细胞诱导为M0→M1→M2的一些关键细节 图片引用自:https://www.caltagmedsystems.co.uk/information/pma-standard-reagent-for-thp1-cell-differentiation/ 为了成功地将THP-1细胞诱导成M0巨噬细胞,并随后将其极化为M1或M2巨噬细胞,需要考虑以下几个关键因素和方法: THP-1细胞向M0巨噬细胞的分化 1.PMA诱...
1. Optimized THP-1 differentiation is required for...; Park et al, Inflamm Res. 2007, 56, 45. 2. p38α MAP kinase serves cell type-specific inflammatory functions ...; Kim et al, Nat Immunol 2008, 9, 1019. 3. PDTC is a potent antioxidant...; Zhu et al, FEBS Letters 2002, 532...
单核细胞THP-1用PMA诱导后，由悬浮式生长变成贴壁生长，由圆形变成不规则形态，体积进一步增大，细胞浆疏...
• 6 种可选荧光通道 + 透射光 • 环境控制模块系统通过CellReporterXpress™ 成像和分析软件进行控制SpectraMax® iD5 多功能酶标仪是用来测量吸光度，计算浓度和 EC50 值。参考文献 1 Optimized THP-1 differentiation is required for�;Parketal,InflammRes.2007, 56, 45.2 p38α MAP kinase serves ...
[2]Bile acid metabolites control TH17 and Treg cell differentiation.[J].Hang Saiyu,Paik Donggi,Yao Lina,Kim Eunha,Trinath Jamma,Lu Jingping,Ha Soyoung,Nelson Brandon N,Kelly Samantha P,Wu Lin,Zheng Ye,Longman Randy S,Rastinejad Fraydoon,Devlin A Sloan,Krout Michael R,Fischbach Michael A,...
Carmen Feinweber, Sonja Luckhardt, etc. THP-1 and human peripheral blood mononuclear cell-derived ...
这为作者后续研究特定基因在THP-1细胞中的 inacuteandchronicmyeIoidIeukemiacelllines.Genomics 功能奠定了基础 。 ProteomicsBioinofrmatics.2014;12(2):79-91. 【11】TrojanowiczB.UlrichC,SeibertE。eta1.UremicConditions DriveHumanMonocytestoPro-AtherogenicDifferentiation 孝贡盾 设计、实施、评估者均为本文...
系统通过CellReporterXpress™成像和分析软件进行控制 SpectraMax® iD5多功能酶标仪是用来测量吸光度,计算浓度和EC50 值 参考文献: 1 Optimized THP-1 differentiation is required for;Parketal,InflammRes.2007,56, 45. 2 p38α MAP kinase serves cell type-specific inflammatory functions; Kim et al...
THP-1人急性单核细胞白血病细胞是从一名患有急性单核细胞白血病的1岁小男孩的外周血中分离得到的,自1980年建系以来,THP-1细胞被广泛用于单核细胞和巨噬细胞相关的机制、信号通路以及营养和药物运输等研究中。相对于U937、HL-60、ML-2等白血病细胞系,THP-1更有类似人原代单核细胞的形态和功能特征(包括细胞分化标...
Treatment with low-dose PMA with RANKL and MCSF failed to induce THP-1 cell differentiation into osteoclasts. PMA alone, or a combination of RANKL and MCSF alone, is insufficient to stimulate THP-1 cell differentiation into osteoclasts. In the present study, a reliable human osteoclast model ...