将THP-1细胞诱导为M0→M1→M2的一些关键细节 图片引用自:https://www.caltagmedsystems.co.uk/information/pma-standard-reagent-for-thp1-cell-differentiation/ 为了成功地将THP-1细胞诱导成M0巨噬细胞,并随后将其极化为M1或M2巨噬细胞,需要考虑以下几个关键因素和方法: THP-1细胞向M0巨噬细胞的分化 1.PMA诱...
图片引用自：https://www.caltagmedsystems.co.uk/information/pma-standard-reagent-for-thp1-cell-differentiation/ 在实际实验过程中，THP-1的状态是影响诱导效果的最大因素。此外，由于诱导时间较长，随时关注诱导过程中的细胞状态，也十分关键。针对这一难点，富衡推出THP-1成巨噬细胞诱导试剂盒，试剂盒中不仅包括...
具体诱导过程见下图 图片引用自:https://www.caltagmedsystems.co.uk/information/pma-standard-reagent-for-thp1-cell-differentiation/ 在实际实验过程中,THP-1的状态是影响诱导效果的最大因素。此外,由于诱导时间较长,随时关注诱导过程中的细胞状态,也十分关键。针对这一难点,富衡推出THP-1成巨噬细胞诱导试剂盒,试...
Iron chelator induces THP-1 cell differentiation potentially by modulating intracellular glutathione levels[J] . Geom-Seog Seo,Sung-Hee Lee,Suck-Chei Choi,Eun-Young Choi,Hyun-Mee Oh,Eun-Ju Choi,Do-Sim Park,Sang-Wook Kim,Tae-Hyeon Kim,Yong-Ho Nah,Soonhag Kim,Sang-Hyun Kim,Sun-Hae You,...
Phorbol 12-myristate 13-acetate (PMA) is commonly used to activate protein kinase C (PKC) and a Standard Reagent for THP1 cell differentiation into macrophages-type cells.
Carmen Feinweber, Sonja Luckhardt, etc. THP-1 and human peripheral blood mononuclear cell-derived ...
系统通过CellReporterXpress™成像和分析软件进行控制 SpectraMax® iD5多功能酶标仪是用来测量吸光度,计算浓度和EC50 值 参考文献: 1 Optimized THP-1 differentiation is required for;Parketal,InflammRes.2007,56, 45. 2 p38α MAP kinase serves cell type-specific inflammatory functions; Kim et al...
1. Optimized THP-1 differentiation is required for...; Park et al, Inflamm Res. 2007, 56, 45. 2. p38α MAP kinase serves cell type-specific inflammatory functions ...; Kim et al, Nat Immunol 2008, 9, 1019. 3. PDTC is a potent antioxidant...; Zhu et al, FEBS Letters 2002,...
这为作者后续研究特定基因在THP-1细胞中的 inacuteandchronicmyeIoidIeukemiacelllines.Genomics 功能奠定了基础 。 ProteomicsBioinofrmatics.2014;12(2):79-91. 【11】TrojanowiczB.UlrichC,SeibertE。eta1.UremicConditions DriveHumanMonocytestoPro-AtherogenicDifferentiation 孝贡盾 设计、实施、评估者均为本文...
本细胞株相关资料参考ATCC (American Type Culture Collection)、DSMZ (German Collection of Microorganisms and Cell Cultures)、JCRB (Japanese Collection of Research Bioresources Cell Bank)、Cellosaurus (Swiss Institute of Bioinformatics)等网站信息,并结合碧云天实际培养信息综合而成。由于细胞培养的条件、代数等因...