1 Optimized THP-1 differentiation is required for�;Parketal,InflammRes.2007, 56, 45.2 p38α MAP kinase serves cell type-specific inflammatory functions�; Kim et al, Nat Immunol. 2008, 9, 1019.3 PDTC is a potent antioxidant�; Zhu et al, FEBS Letters 2002, 532, 80.4 Anti-Infla...
参考文献: 1 Optimized THP-1 differentiation is required for;Parketal,InflammRes.2007,56, 45. 2 p38α MAP kinase serves cell type-specific inflammatory functions; Kim et al, Nat Immunol. 2008,9, 1019. 3 PDTC is a potent antioxidant; Zhu et al, FEBS Letters 2002,532, 80. 4...
The in vitro THP-1 monocyte differentiation into polarized macrophages was used to study the effects of both M1 and M2 type populations of macrophages on melanoma cells (Smith et al., 2014; Tsuchiya et al., 1980). M1 type macrophages are classically thought to be tumour suppressing as ...
These findings suggest a modified PMA differentiation protocol can enhance macrophage differentiation of THP-1cells and identify increased numbers of mitochondria and lysosomes,resistance to apoptosis and the potency of TLR2responses as important discriminators of the level of macrophage differentiation for ...
Monocytic differentiation can be induced with the phorbol ester 12-O-tetradecanoylphorbol-13-acetate (TPA). Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following...
比较不同诱导条件下的细胞THP-1细胞
(2010) published a protocol for THP-1 cell differentiation, showing that THP-1 cells represent a simplified model to study monocyte-macrophage polarization (Qin 2012). U937 is a pro-monocytic, human myeloid leukaemia cell line and was isolated from the histiocytic lymphoma of a 37 year old ...
Phorbol 12-myristate 13-acetate (PMA) is commonly used to activate protein kinase C (PKC) and a Standard Reagent for THP1 cell differentiation into macrophages-type cells.
Optional: PMA-induced THP-1 Differentiation Following Phorbol 12-myristate 13-acetate (PMA) treatment, THP1‑Dual™ cells are more sensitive to IFN-inducers, such as transfected Poly(dA:dT), while the response to certain TLR ligands, such as Pam3CSK4, is diminished. Note: PMA treatment ...
These findings suggest a modified PMA differentiation protocol can enhance macrophage differentiation of THP-1 cells and identify increased numbers of mitochondria and lysosomes, resistance to apoptosis and the potency of TLR2 responses as important discriminators of the leve...