1 Optimized THP-1 differentiation is required for�;Parketal,InflammRes.2007, 56, 45.2 p38α MAP kinase serves cell type-specific inflammatory functions�; Kim et al, Nat Immunol. 2008, 9, 1019.3 PDTC is a potent antioxidant�; Zhu et al, FEBS Letters 2002, 532, 80.4 Anti-Infla...
参考文献: 1 Optimized THP-1 differentiation is required for;Parketal,InflammRes.2007,56, 45. 2 p38α MAP kinase serves cell type-specific inflammatory functions; Kim et al, Nat Immunol. 2008,9, 1019. 3 PDTC is a potent antioxidant; Zhu et al, FEBS Letters 2002,532, 80. 4...
比较不同诱导条件下的细胞THP-1细胞
(2010) published a protocol for THP-1 cell differentiation, showing that THP-1 cells represent a simplified model to study monocyte-macrophage polarization (Qin 2012). U937 is a pro-monocytic, human myeloid leukaemia cell line and was isolated from the histiocytic lymphoma of a 37 year old ...
2.1 Differentiation of THP-1 macrophages THP-1 cells in the monocyte state can be differentiated into a macrophage-like phenotype using either phorbol-12-myristate-13-acetate (PMA), 1α, 25-dihydroxyvitamin D3 (vD3) or macrophage colony-stimulating factor (M-CSF). Although the latter stimulus ...
These findings suggest a modified PMA differentiation protocol can enhance macrophage differentiation of THP-1 cells and identify increased numbers of mitochondria and lysosomes, resistance to apoptosis and the potency of TLR2 responses as important discriminators of the level of macrophage differentiation ...
THP-1 cells were cultured for 24 h in medium containing 50 ng/mL PMA (Sigma-Aldrich, USA), which was used to induce THP-1 monocyte differentiation into macrophages. Adherent cells were then infected with BCG at an MOI of 10. Construction and transfection of DUSP1 small interfering RNAs ...
Monocytes are the most prominent environment-sensing immune cells in circulation, skilled to shape their microenvironments via cytokine secretion and further differentiation. Both the circulating monocyte subset distribution and the blood plasma EV pattern are characteristic for preeclampsia, a pregnancy ...
Contamination of this cell line may activate TLRs resulting in differentiation of the monocytes and activation of the reporter gene. • Test Medium: RPMI 1640, 2 mM L-glutamine, 25 mM HEPES, 10% (v/v) heat-inactivated fetal bovine serum (FBS), 100 U/ml penicillin, 100 µg/ml ...
S2). These results suggest that complete THP-1 monocyte differentiation into MDMs occurs at 48 h. This observation was used as the optimum time to select MDMs for experiments. Figure 1 PMA induced differentiation of THP-1 monocytes into adherent macrophage-like cells was observed under the light...