加入终浓度为100ng/ml的PMA，24孔培养板中加入1ml细胞悬液，诱导分化24h，替换成新鲜RPMI 1640完全培养...
我们提出了多参数细胞分析的结果，使用表型成像考察巨噬细胞形成和低体积ELISA 分析细胞因子的分泌，以评估药理学化合物对炎症反应的影响。PMA 和 LPS刺激 THP-1 细胞 48 小时。PMA 和 LPS 激活 THP-1 细胞后，IL-8、IL-1b 和 TNF-a升高。为了评估抗炎化合物，细胞在激活前分别接受激酶抑制剂 SB202190 和 P...
These findings suggest a modified PMA differentiation protocol can enhance macrophage differentiation of THP-1cells and identify increased numbers of mitochondria and lysosomes,resistance to apoptosis and the potency of TLR2responses as important discriminators of the level of macrophage differentiation for ...
THP-1 monocytes were differentiated into macrophages using PMA. PMA-treated THP-1 cells were co-cultured with human A549 cells culture supernatant. SPP1 ... Y Zhang,W Du,Z Chen,... - 《Experimental Cell Research》 被引量: 1发表: 2017年 Protocols for Co-Culture Phenotypic Assays with Brea...
This research optimized the induction method on THP-1 cell differentiation from three aspects and delineated the transcriptomic profile of PMA-induced THP-1 cells, laying a foundation for the construction method of cell model and for the functional study of macrophage....
比较不同诱导条件下的细胞THP-1细胞
人单核细胞系THP-1可以通过佛波醇12-十四酸酯13-乙酸酯(PMA)分化成巨噬细胞,并通过LPS活化。活化的THP-1细胞改变形态,分泌炎性细胞因子。监测细胞因子的表达水平是炎症细胞模型的重要生理指标。我们提出了多参数细胞分析的结果,使用表型成像考察巨噬细胞形成和低体积ELISA分析细胞因子的分泌,以评估药理学化合物对炎症...
Phorbol 12-myristate 13-acetate (PMA) is commonly used to activate protein kinase C (PKC) and a Standard Reagent for THP1 cell differentiation into macrophages-type cells.
14.6 Differentiation of THP-1 and U937 Monocytes into Macrophages As already mentioned before, nearly all THP-1 cells start to adhere to culture plates and differentiate into macrophages after exposure to PMA. Also U937 pro-monocytes differentiate into mature monocytes or into macrophages upon PMA-tr...
A 24 h incubation in the presence of 150 nM PMA followed by 24 h in control medium was finally selected as differentiation protocol. Cells became adherent and the expression of recognized macrophage markers, CD68 (cluster of differentiation 68) [16], CD71 [17] and CD36 [18], analyzed by ...