加入终浓度为100ng/ml的PMA，24孔培养板中加入1ml细胞悬液，诱导分化24h，替换成新鲜RPMI 1640完全培养...
多参数炎症试验的方案如下。经刺激后，分化的 THP-1 细胞贴附于平板，分泌上调的细胞因子。THP-1 分化导致的表型变化如图 2 所示。PMA 和 LPS 刺激细胞因子分泌增加如图 3 所示。• THP-1 细胞每 96 孔铺 2 万个细胞，孵育48 小时。然后用 PMA 和 LPS 混合刺激24 小时 (0-5 pg/mL PMA, 0-100 pg...
THP-1 monocytes were differentiated into macrophages using PMA. PMA-treated THP-1 cells were co-cultured with human A549 cells culture supernatant. SPP1 ... Y Zhang,W Du,Z Chen,... - 《Experimental Cell Research》 被引量: 1发表: 2017年 Protocols for Co-Culture Phenotypic Assays with Brea...
These findings suggest a modified PMA differentiation protocol can enhance macrophage differentiation of THP-1cells and identify increased numbers of mitochondria and lysosomes,resistance to apoptosis and the potency of TLR2responses as important discriminators of the level of macrophage differentiation for ...
Phorbol 12-myristate 13-acetate (PMA) is commonly used to activate protein kinase C (PKC) and a Standard Reagent for THP1 cell differentiation into macrophages-type cells.
比较不同诱导条件下的细胞THP-1细胞
Gatto, F.; Cagliani, R.; Catelani, T.; Guarnieri, D.; Moglianetti, M.; Pompa, P.; Bardi, G. PMA-Induced THP-1 Macrophage Differentiation is Not Impaired by Citrate-Coated Platinum Nanoparticles. Nanomaterials 2017, 7, 332. [CrossRef] [PubMed]...
多参数炎症试验的方案如下。经刺激后,分化的THP-1细胞贴附于平板,分泌上调的细胞因子。THP-1分化导致的表型变化如图2所示。PMA和LPS刺激细胞因子分泌增加如图3所示。 • THP-1细胞每96孔铺2万个细胞,孵育48小时。然后用PMA和LPS混合刺激24小时(0-5pg/mL PMA,0-100pg/mL LPS);均来自Sigma。
Chanput W, Mes JJ, Wichers HJ (2014) THP-1 cell line: an in vitro cell model for immune-modulation approach. Int Immunopharmacol. doi:10.1016/j.intimp.2014.08.002 Chun EM, Park YJ et al (2001) Expression of the apolipoprotein C-II gene during myelomonocytic differentiation of human leuk...
Optional: PMA-induced THP-1 Differentiation Following Phorbol 12-myristate 13-acetate (PMA) treatment, THP1‑Dual™ cells are more sensitive to IFN-inducers, such as transfected Poly(dA:dT), while the response to certain TLR ligands, such as Pam3CSK4, is diminished. Note: PMA treatment ...