1 Optimized THP-1 differentiation is required for�;Parketal,InflammRes.2007, 56, 45. 2 p38α MAP kinase serves cell type-specific inflammatory functions�; Kim et al, Nat Immunol. 2008, 9, 1019. 3 PDTC is a potent antioxidant�; Zhu et al, FEBS Letters 2002, 532, 80. 4 ...
分化的 THP-1 细胞已被广泛用于体外巨噬细胞模型研究巨噬细胞参与炎症反应。人单核细胞系 THP-1 可以通过佛波醇 12-十四酸酯 13-乙酸酯 (PMA) 分化成巨噬细胞，并通过 LPS 活化。活化的 THP-1 细胞改变形态，分泌炎性细胞因子。监测细胞因子的表达水平是炎症细胞模型的重要生理指标。我们提出了多参数细胞分析的...
将THP-1细胞诱导为M0→M1→M2的一些关键细节 图片引用自:https://www.caltagmedsystems.co.uk/information/pma-standard-reagent-for-thp1-cell-differentiation/ 为了成功地将THP-1细胞诱导成M0巨噬细胞,并随后将其极化为M1或M2巨噬细胞,需要考虑以下几个关键因素和方法: THP-1细胞向M0巨噬细胞的分化 1.PMA诱...
1. Optimized THP-1 differentiation is required for...; Park et al, Inflamm Res. 2007, 56, 45. 2. p38α MAP kinase serves cell type-specific inflammatory functions ...; Kim et al, Nat Immunol 2008, 9, 1019. 3. PDTC is a potent antioxidant...; Zhu et al, FEBS Letters 2002,...
图片引用自:https://www.caltagmedsystems.co.uk/information/pma-standard-reagent-for-thp1-cell-differentiation/ 在实际实验过程中,THP-1的状态是影响诱导效果的最大因素。此外,由于诱导时间较长,随时关注诱导过程中的细胞状态,也十分关键。针对这一难点,富衡推出THP-1成巨噬细胞诱导试剂盒,试剂盒中不仅包括各阶...
study of the macrophagic differentiation. In fact, various physical and chemical agents can induce these cell lines to differentiate into mature mono- cytes. Differentiation is characterized by perma- nent alterations in a variety of cellular parameters, ...
Monocytic differentiation can be induced with the phorbol ester 12-O-tetradecanoylphorbol-13-acetate (TPA). Culture Method Doubling Time 26~70 hrs Methods for Passages Split cells to new flask with fresh medium. No need to treat them enzymatically to detach them from the surface of the ...
Here, we provide a dataset on the optimized conditions for the THP-1 differentiation and the induction of NO/iNOS signaling under inflammatory stimulus. The human monocytic cells were differentiated into adherent macrophage-like phenotype by phorbol-12-myristate-13-acetate (PMA) stimulation under ...
CommentsThe cells are phagocytic (for both latex beads and sensitized erythrocytes) and lack surface and cytoplasmic immunoglobulin. Monocytic differentiation can be induced with the phorbol ester 12-O-tetradecanoylphorbol-13-acetate (TPA). Culture Method ...
参考文献:1. Tsuchiya S, et al. Establishment and characterization of a human acute monocytic leukemia cell line (THP-1). Int J Cancer. 1980; 26(2):171-6.2. Rössner S, et al. Differentiation of human monocytic cell lines transfected with MCSF-receptor cDNA is associated with c-fms ...