方法 首先用 PMA梯度剂量(0,25,50,100,200,400ng/ml)作用于 THP 1细胞 24h,通过 CCK 8法检测细胞活力,再分别采用 0,10,50ng/mlPMA体外刺激 THP 1细胞 24h、48h,流式细胞术检测细胞表面标志物 CD11b、CD14。 结果 梯度 PMA剂量(0-100ng/ml)对细胞活力没有影响(P>005)。10ng/ml、50ng/mlPMA处理...
THP-1更易在实验室中培养和扩增，且具有更稳定的基因背景，不存在PBMC的个体差异性问题，利于实验结果的...
LPS 致病作用的关键在于 其通过激活 Toll 样受 体 4 (toll like receptor 4,TLR4)跨膜信号通路, 活化以单核/巨噬细胞为代表的宿主免疫细胞,促进细胞因子和炎症介质的过度释放, 引 起全身失控性炎症级联反应,TNF-α、IL-6 在·论著·[文章编号]1000-8861(2013)02-0121-05分化诱导剂 PMA 对 THP-1 源性...
PMA诱导人单核细胞白血病细胞株THP-1细胞表面标志物CD14和CD11b的表达
PMA-induced THP-1 macrophage differentiation is not impaired by citrate-coated platinum nanoparticles. Nanomaterials (Basel) 7, 332. (1-10).Gatto, F.; Cagliani, R.; Catelani, T.; Guarnieri, D.; Moglianetti, M.; Pompa, P.; Bardi, G. PMA-Induced THP-1 Macrophage Differentiation is ...
两种佛波酯方案可诱导THP-1产生不同表型的巨噬细胞以及后续极化亚型, 需根据实验目 的选择合适的诱导方案。 关键词 THP-1; 巨噬细胞; 极化; 佛波酯 The Effects of Two Different PMA Induction on M1 and M2 Subtypes Related Genes Expression Profile in Differentiation and Polarization of THP-1 Derived ...
Phorbol 12-myristate 13-acetate (PMA) 属于佛波酯类天然产物,是一种 PKC、SphK、NF-κB 的激活剂。Phorbol 12-myristate 13-acetate 可诱导 THP1 细胞分化。 规格价格库存数量 1 mg¥ 436现货 5 mg¥ 1,050现货 10 mg¥ 1,770现货 25 mg¥ 3,730现货 ...
Analysis of cell morphology, cell adhesion, expression of surface markers and phagocytic capacity illustrated that while both VD3 and PMA stimulation induced macrophage differentiation important differences existed in comparison to MDM. A protocol in which THP1 cells were activated with PMA then rested ...
The proteomic analysis of anti-inflammatory effects of geniposidic acid in LPS induced THP1 monocytic cells People's health is adversely impacted by diseases caused by abnormal inflammation; therefore, more effective treatments as well as research into the underl... G Wang,Y Qing,Q Zhang,......
cholesterylacyltransferase1(ACAT1)duringthedifferentiationofTHP一1monocytesintomacrophagesbyphorbolesters(PMA).MethodsTheTHP一1humanmonocyticleukemiaeelIline(THP一1)wasculturedinvitro.ThedifferentiationofTHP一1cellsintomacrophageswasinducedbyPMA.TheACAT1enzymeactivitywasmeasuredbyquantifyingtheincorporationof[1一c]...