Importantly, we reveal an important role of the PI3K/AKT pathway in PMA-induced THP1 cell differentiation. The identified DEGs and pathways may facilitate further study of the detailed molecular mechanisms of THP1 differentiation. Thus, our results provide numerous potential therapeutic targets for ...
方法 首先用 PMA梯度剂量(0,25,50,100,200,400ng/ml)作用于 THP 1细胞 24h,通过 CCK 8法检测细胞活力,再分别采用 0,10,50ng/mlPMA体外刺激 THP 1细胞 24h、48h,流式细胞术检测细胞表面标志物 CD11b、CD14。 结果 梯度 PMA剂量(0-100ng/ml)对细胞活力没有影响(P>005)。10ng/ml、50ng/mlPMA处理...
Phorbol 12-myristate 13-acetate (PMA) is commonly used to activate protein kinase C (PKC) and a Standard Reagent for THP1 cell differentiation into macrophages-type cells.
虽然是目前比较通用的巨噬细胞模型,但既往文献关于PMA诱导THP-1模型中,不同PMA诱导条件对细胞表面标志物CD11b、CD14表达等尚无系统的比较研究。基于此,本研究拟对不同浓度PMA作用THP-1细胞后,其CD11b、CD14的表达进行分析,初步考察了PMA诱导THP-1细胞分化的条件,为后续相关实验提供实验依据。 1 材料与方法 1.1 ...
摘要: In this work, the influence of different substrate adhesion duringphorbol-12-myristate-13-acetate (PMA)-induced differentiation of THP-1monocytic cell line was studied. In particular, by morphocytochemical andcytometric approaches, the influence of ty...关键词:...
Phorbol 12-myristate 13-acetate induces THP1 cell differentiation. 靶点活性 PKC:11.7 nM (EC50) 体外活性 方法:球体培养的人类黑色素瘤细胞 WM 系列用 Phorbol 12-myristate 13-acetate (50 ng/mL) 处理 3 天,使用 MTS 方法检测细胞生长情况。结果:Phorbol 12-myristate 13-acetate 促进黑色素瘤细胞增殖,...
Analysis of cell morphology, cell adhesion, expression of surface markers and phagocytic capacity illustrated that while both VD3 and PMA stimulation induced macrophage differentiation important differences existed in comparison to MDM. A protocol in which THP1 cells were activated with PMA then rested ...
1monocytesintomacrophagesbyphorbolesters(PMA).MethodsTheTHP一1 humanmonocyticleukemiaeelIline(THP一 1)wasculturedinvitro.ThedifferentiationofTHP一1cellsintomacrophageswas inducedbyPMA.TheACAT1enzymeactivitywasmeasuredbyquantifyingtheincorporatio nof[1一c]oleoylCoAintochol— esterylesters.TheexpressionofACAT1pr...
ObjectivesTo analyse proliferation, differentiation and apoptosis in THP-1 cells after stimulation with phorbol 12-myristate 13-acetate (PMA) and retinoic acid (RA).Materials and methodsPMA and RA were used in a three-step-procedure: (i) treatment with 6, 30, 60 nm PMA, that induced initial...
(a specific AMPK inhibitor) reduced MMP-9 and EMMPRIN expression levels in PMA-induced THP-1 cell differentiation, which suggested that activation of the AMPKα pathway may be involved in the regulation of EMMPRIN and MMP-9 expression in PMA-induced macrophages (20). Moreover, a number of ...