THP-1mtDNA copy numberMonocytes are differentiated into macrophages. In this study, mitochondrial DNA copy number (mtDNAcn) levels and downstream events such as the expression of respiratory chain mRNAs were investigated during the phorbol 12-myristate 13-acetate (PMA)-induced differentiation of ...
Understanding how immune cells such as macrophages interact with cancer cells is of increasing interest, as cancer treatments move towards combining both targeted- and immuno- therapies in new treatment regimes. This protocol is using THP-1 cells, a human leukemia monocytic cell line that can be ...
OBJECTIVES: The differentiation of THP-1 monocytes into macrophages is mainly conducted at a phorbol 12-myristate 13-acetate (PMA) concentration of 10-400 ng/ml. However, this concentration might be high enough to upregulate the expressions of some genes in differentiated macrophages, which could ...
cohol (PVA), with U937 and THP-1 cells. Meas- urement of differentiation into macrophages will be analyzed by means of biochemical and mor- phological assays. MATERIALS AND METHODS Cell culture - THP-1 cells, leukemic cell line de- ...
Ding Q, Jin T, Wang Z, Chen Y (2007) Catalase potentiates retinoic acid-induced THP-1 monocyte differentiation into macrophage through inhibition of peroxisome proliferator-activated receptor gamma. J Leukoc Biol 81:1568–1576. doi: 10.1189/jlb.1106672...
total amount of GLUT-1 protein did not increase upon rESAT-6 stimulation (Fig. 1E, see supplementary Fig. S1 online) and it was only the translocation of the protein from the cytosol to the cell membrane that was enhanced by rESAT-6 to increase glucose uptake by THP-1 macrophages. ...
Abstract We have been interested in the immunomodulatory effect, to promote differentiation of the human monocytic cell line THP-1 to macrophages of EM-A. We chemically modified EM-A in order to obtain derivatives with stronger promoting activity of monocyte to macrophage differentiation and no ...
We have identified and characterized the synthesis of proteoglycans and related proteins, some not previously known to be associated with macrophages. Proteoglycan extracts of [35S]sulfate- and 35S-trans amino acid-radiolabeled culture media from THP-1 monocytes induced to differentiate by treatment ...
isolating relatively pure populations of non-differentiated monocytes and THP-1-derived macrophages [49]. Isolation of homogeneous cell populations is particularly appealing for the study of tRNA gene dynamics during cellular differentiation, as tDNAs, which are essential for biosynthesis, are likely to...
5) human monocytic-leukemia cells (THP-1) 人单核巨噬细胞6) monocyte-derived macrophages 单核源巨噬细胞 1. Objective To investigate the clinical significance of matrix metalloproteinase-9 (MMP-9) and tissue factor (TF) secreted by cultured monocyte-derived macrophages (HMDM) from patients ...