THP-1 differentiation and coculture system To be differentiated into adherent macrophages, THP-1 cells (5 × 105/well) were seeded into 6-well plates in the presence of 100 ng/ml of phorbol 12-myristate 13-acetate (PMA, Sigma) for 24 h. The differentiated macrophages were named as ...
14.6Differentiation of THP-1 and U937 Monocytes into Macrophages As already mentioned before, nearly all THP-1 cells start to adhere to culture plates and differentiate into macrophages after exposure to PMA. Also U937 pro-monocytes differentiate into mature monocytes or into macrophages upon PMA-trea...
In particular, DC differentiation and maturation in 3D were evaluated comparing the conventional TCP-based culture condition. Method: THP-1 cells were encapsulated in poly(ethylene glycol) (PEG) hydrogel via thiol-ene photocrosslinking with non-degradable or proteolytically degr...
The up-regulation of UAE1 in mCRP stimulated monocytes may also be a sign of the induction of the macrophagic differentiation process induced by mCRP, as a crucial role for UAE1 in the differentiation process and the associated nucleotide excision repair has been recently described [25]. Both ...
THP-1 cells resemble primary monocytes and macro- phages in morphology and differentiation properties. THP-1 cells show a large, round single-cell morphology and express distinct monocytic markers. Nearly all THP-1 cells start to adhere to culture plates and differentiate into macrophages after ...
The cells containing 2.5~3 × 105 cells/cm2 in 6-well plates were treated with 200 nM of phorbol 12-myristate 13-acetate (PMA) to induce the differentiation of THP1 monocytes into macrophages. After 16 h of treatment, PMA was removed and the cells were washed twice with ...
【摘要】 目的：探讨即早基因ｃ ｆｏｓ在ＴＨＰ １巨噬细胞亚型极化过程中的表达变化。方法：运用ＰＭＡ刺激诱导 ＴＨＰ １单核细胞极化为巨噬细胞，观察ｃ ｆｏｓ在单核细胞极化过程中的表达变化；在ＰＭＡ刺激的基础上，分别运用 ＬＰＳ和ＩＬ ４诱导ＴＨＰ １巨噬细胞向Ｍ１及Ｍ２亚型极化，实时定量Ｐ...
ObjectivesTo analyse proliferation, differentiation and apoptosis in THP-1 cells after stimulation with phorbol 12-myristate 13-acetate (PMA) and retinoic acid (RA).Materials and methodsPMA and RA were used in a three-step-procedure: (i) treatment with 6, 30, 60 nm PMA, that induced initial...
Differentiated THP-1 cells were Figure 5. ERK activity is required for the TCDD-induced TNF-α expression. (A) After differentiation into macrophages with PMA, THP-1 cells were cul- tured in RPMI-1640 containing 0.5% FBS for 24 h before stimulation with TCDD (75 nM). ERK activation was...
Oncogene (2004) MLL-AF9 oncogene expression affects cell growth but not terminal differentiation and is downregulated during monocyte–macrophage maturation in AML-M5 THP-1 cells Andrea Pession Vincenzo Martino Guido Paolucci Oncogene (2003)Sections...