1. 取出少量细胞悬液进行细胞计数及活力检测,当细胞密度达到1×106 cells/mL 时,进行细胞传代培养。 2. 取足量细胞加入到盛有新鲜培养基的培养瓶中,将细胞密度维持在2-3×105cells/mL。 3. 将培养瓶竖直放置于含有5%CO2的37℃恒温培养箱中培养。如果细胞达到传代培养的密度,则进行传代培养。 培养基换液: 每...
THP-1-LUC(人单核细胞白血病细胞-荧光素酶标记(STR鉴定正确)) 白血病 细胞 厦门逸漠生物科技有限公司 2年 查看详情 ￥1800.00/瓶 上海 THP-1(人单核细胞白血病)(STR鉴定正确) 1×10⁶cells/T25培养瓶 24小时发货 少货必赔 破损包赔 天津睿创生物科技有限公司 2年 展开更多 百度爱采购温馨提示 以...
1. Comparative proteomics study of the THP-1 cells engulfing Coxiella burnetti; THP-1细胞吞噬贝氏柯克斯体后的比较蛋白质组学研究 2. Effects of PMA on CD147 expression in cultured supernatants of in vitro cultured THP-1 cells and on monocytes of RA patients; PMA对THP-1细胞和RA患者单核细胞...
THP-1 cells: The Human acute monocytic leukemia cell lineMy Publications
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THP-1巨噬细胞 1. In order to study the effects of LDL and oxLDL on expression of PCSK9 and LDLR in THP-1 macrophages and find the relationship between them. 分别用0mg/L、10mg/L、20mg/L、30mg/L的LDL和0mg/L、10mg/L、20mg/L、30mg/L的oxLDL处理THP-1巨噬细胞,油红O染色检测细胞荷...
THP-1 cells as a model for human monocytes The involvement of cytosolic free calcium (Ca2+) as a second messenger in lipopolysaccharide (LPS)-activated cellular pathways remains controversial. Using human monocyte-like THP-1 cells stably transfected with glycosylphosphatidylinosi... H Bosshart,M ...
1. Objective:To determine the human neutrophil peptide-1(HNP-1) gene expression in low density lipoprotein(LDL) oxidation mediated by THP-1 cells and construct the cloning vector of human HNP-1. 方法:提取人单核细胞系(THP-1)的总RNA,逆转录聚合酶链反应(RT-PCR)法获得cDNA,采用pBS-T克隆HNP...
The role of human T cells in the induction and regulation, upon cell/cell contact, of inflammatory responses by monocytic cells was investigated. The production of interleukin (IL)-1beta and IL-1 receptor antagonist (IL-1Ra) by the monocytic THP-1 cell line was measured upon contact with ei...
F. Chen, "The effects of two different ganoderma species (Lingzhi) on gene expression in human monocytic THP-1 cells," Nutrition and Cancer, vol. 62, no. 5, pp. 648-658, 2010.Cheng, C.H.; Leung, A.Y.; Chen, C.F. The effects of two ...