THP-1 cells form foam cells in response to coculture with lipoproteins but not platelets. J Lipid Res. 1991; 32 :35–43. [ PubMed ]Banka CL, Black AS, Dyer CA, and Curtiss LK: THP- 1 cells form foam cells in response to coculture with lipoproteins but not platelets. J Lipid Res...
Monocytes are circulatory precursor cells from myeloid origin that can develop into macrophages or dendritic cells upon migration from the blood stream to tissues. Both macrophages and dendritic cells are professional antigen-presenting cells. Monocytes
The inhibitory rate of the secretion of proMMP-9, the activated MMP-9 and proMMP-2 in the differentiated THP-1 cells was 52.90%, 53.79% and 47.80%, respectively. The inhibitory rate of invasive potential in the undifferentiated cells and the differentiated THP-1 cells was 41.82 % and ...
Full size image In vivo study of the effects of BCG induced inflammation and the therapeutic potential of targeting DUSP1 Three small interfering RNA sequences were designed and synthesized based on the murine-derived DUSP1 sequence, and siRNA-DUSP1 was transfected into RAW264.7 cells. The knockdo...
ELISA of TNF-α produced by THP-1 cells challenged by Sterne and Δ Sterne over 8 h. Full size image This large cellular TNF-α response to toxigenic spores compared to a lower one in the nontoxigenic spore-challenge is an interesting observation, suggesting that the issue of cytokine suppre...
When an autophagic stimulus occurs, LC3-I is conjugated to the phosphatidyletha- nolamine (PE) to be converted into form II (LC3-II) which associates with the autophagosomal membrane. First, we infected THP-1 cells with HSV-1 at the multiplicity of infection (MOI) 50, and we evaluated ...
(secreted embryonic alkaline phosphatase) activities, respectively. We observe an impact of the IRF-7 knock-out on NF-κB-mediated responses, and no effect on IRF-mediated responses, in THP1-Dual™ KO-IRF7 cells upon incubation with TLR agonists when compared to theTHP1-Dual™ cells(see...
(Fig.3). Under physiological shear-stress conditions, non-activated monocytic cells did not adhere to ICAM-1 in relevant numbers, either with or without ADP stimulation (THP-1 monocytes/cm2: THP-1 monocytes vs. THP-1 monocytes + ADP: 16.9 + 6.3 vs. 19.9 ± 6.5,p >...
Intercellular communication via extracellular vesicles (EVs) and their target cells, especially immune cells, results in functional and phenotype changes that consequently may play a significant role in various physiological states and the pathogenesis of immune-mediated disorders. Monocytes are the most pr...
Cells were cultivated at a density of approximately 3–8 × 105 cells/mL to maintain exponential growth up to passage 25 (~ 3 months) and were tested and verified as mycoplasma-free34. Cell number was calculated using Trypan blue exclusion (1:1 cells: Trypan blue, Sigma) to ensure...