Monocytes are circulatory precursor cells from myeloid origin that can develop into macrophages or dendritic cells upon migration from the blood stream to tissues. Both macrophages and dendritic cells are professional antigen-presenting cells. Monocytes
Full size image In vivo study of the effects of BCG induced inflammation and the therapeutic potential of targeting DUSP1 Three small interfering RNA sequences were designed and synthesized based on the murine-derived DUSP1 sequence, and siRNA-DUSP1 was transfected into RAW264.7 cells. The knockdo...
When an autophagic stimulus occurs, LC3-I is conjugated to the phosphatidyletha- nolamine (PE) to be converted into form II (LC3-II) which associates with the autophagosomal membrane. First, we infected THP-1 cells with HSV-1 at the multiplicity of infection (MOI) 50, and we evaluated ...
The extract of English walnuts (walnut oil) was dissolved in DMSO and applied to cultured THP-1 MDFC cells (0.5 mg/mL). THP-1 MDFC also were treated with human sera (10%, v:v) taken from subjects in a walnut feeding study. Cholesterol efflux was examined by liquid scintillation counti...
Another player of innate immunity are the dendritic cells (DC), which form an important link between innate and adaptive immunity. DC are relevant in the induction of an immune response as well as in the maintenance of tolerance. Modulation or inhibition of both components, complement and DC, ...
cells which, like THP-1, are resistant to LeTx-induced cytolysis. In the absence of pXO1, the non-toxigenic strain induces early apoptosis consistent with its protective role in several infectious diseases as a form of the host innate immune response. This finding implies that in addition to...
(Fig.3). Under physiological shear-stress conditions, non-activated monocytic cells did not adhere to ICAM-1 in relevant numbers, either with or without ADP stimulation (THP-1 monocytes/cm2: THP-1 monocytes vs. THP-1 monocytes + ADP: 16.9 + 6.3 vs. 19.9 ± 6.5,p >...
form of latent infection. When MAP infects immune cells such as macrophages, inflammatory cytokines such as IL-1β and various chemokines are expressed during infection [5,6]. In particular, several cytokines involved in the differentiation and maintenance of IL-17-producing T cells, such as IL...
Intra- and extracellular antioxidant capacities of the new water-soluble form of curcumin (NDS27) on stimulated neutrophils and HL-60 cells 2013, Chemico-Biological Interactions Citation Excerpt : It is involved in the inhibition of oxidative response induced by activated PMNs through either ROS scave...
Full size image Fig. 6 Sulforaphane rescued an Aβ1-42-mediated decrease of MerTK expression through inhibiting NF-κB nuclear translocation. a, b THP-1 cells were pretreated with a sulforaphane (5 μM) or b BAY 11-7082 (20 μM) for 30 min and then exposed to Aβ1-42 for 8 h. ...