IRF7 is highly homologous to IRF3, and they can form either homo- or heterodimers, which ultimately lead to IFN production [2]. More details NF-κB and IRF signaling pathways in THP1-Dual™ KO-IRF7 cells Key Features: Verified knockout of the IRF7 gene (PCR, DNA sequencing, and...
Full size image In vivo study of the effects of BCG induced inflammation and the therapeutic potential of targeting DUSP1 Three small interfering RNA sequences were designed and synthesized based on the murine-derived DUSP1 sequence, and siRNA-DUSP1 was transfected into RAW264.7 cells. The knockdo...
1. Introduction The uptake of large amounts of lipids from plasma lipoproteins via endocytosis by macrophages in the artery wall to form lipid-engorged foam cells is an important initiating event in the development of atherosclerosis, a major cause of heart disease. The main lipoprotein implicated ...
The inhibitory rate of the secretion of proMMP-9, the activated MMP-9 and proMMP-2 in the differentiated THP-1 cells was 52.90%, 53.79% and 47.80%, respectively. The inhibitory rate of invasive potential in the undifferentiated cells and the differentiated THP-1 cells was 41.82 % and ...
When an autophagic stimulus occurs, LC3-I is conjugated to the phosphatidyletha- nolamine (PE) to be converted into form II (LC3-II) which associates with the autophagosomal membrane. First, we infected THP-1 cells with HSV-1 at the multiplicity of infection (MOI) 50, and we evaluated ...
You have full access to this open access chapter,Download chapter PDF Keywords THP-1 U937 Cell line Monocyte Macrophage 14.1Origin and Some Features of THP-1 and U937 Cells The THP-1 cell line is a human monocytic leukaemia cell line which was established in 1980 by Tsuchiya et al. (1980...
Myeloid cellsT cellsBackground SAMHD1 counteracts HIV-1 or HIV-2/SIVsmm that lacks Vpx by depleting the intracellular pool of nucleotides in myeloid cells and CD4+ quiescent T cells, thereby inhibiting the synthesis of retroviral DNA by reverse transcriptase. Depletion of nucleotides has been ...
cells which, like THP-1, are resistant to LeTx-induced cytolysis. In the absence of pXO1, the non-toxigenic strain induces early apoptosis consistent with its protective role in several infectious diseases as a form of the host innate immune response. This finding implies that in addition to...
Full size image Effects of siHHLA2 on the migration and invasion of PC cells To clarify the effects of HHLA2 on PC cell metastasis, wound healing and transwell assays have been conducted. As shown in Fig. 6A and B, compared with the NC group, the wound healing rate of siHHLA2-2 grou...
The present study in human monocytic THP-1 cells was designed in order to identify LPS-inducible genes that are down-regulated by the reduced form of coenzyme Q(10) (ubiquinol, Q(10)H(2)). For this purpose, THP-1 cells were incubated with 10μM Q(10)H(2) for 24 hours. ...