IRF7 is highly homologous to IRF3, and they can form either homo- or heterodimers, which ultimately lead to IFN production [2]. More details NF-κB and IRF signaling pathways in THP1-Dual™ KO-IRF7 cells Key Features: Verified knockout of the IRF7 gene (PCR, DNA sequencing, and...
Full size image In vivo study of the effects of BCG induced inflammation and the therapeutic potential of targeting DUSP1 Three small interfering RNA sequences were designed and synthesized based on the murine-derived DUSP1 sequence, and siRNA-DUSP1 was transfected into RAW264.7 cells. The knockdo...
form of fluorescein used as an indicator for ROS in cells. Upon cleavage of the acetate groups by intracellular esterases and oxidation, the non-fluorescent H2DCFDA is converted to the highly fluorescent 2′,7′-dichlorofluorescein (DCF). The chemically reduced and acetylated form of 2′,7′-...
When an autophagic stimulus occurs, LC3-I is conjugated to the phosphatidyletha- nolamine (PE) to be converted into form II (LC3-II) which associates with the autophagosomal membrane. First, we infected THP-1 cells with HSV-1 at the multiplicity of infection (MOI) 50, and we evaluated ...
In conclusion, we provide evidence in THP-1 cells that Q(10)H(2) modulates LPS-induced gene expression. 展开 关键词: coenzyme Q(10 reduced form gene expression lipopolysaccharide regulation microarray analysis THP-1 monocytes DOI: 10.1089/jmf.2010.0080 被引量: 16 ...
D Knockdown of HHLA2 inhibited the activity of AKT/mTOR signaling pathway in AsPC-1 and Capan-2 cells. *p<0.05, **p<0.01, ***p<0.001, ****p<0.0001 Full size image Silencing of HHLA2 in PC cells inhibits M2 polarization of macrophages Finally, effects of HHLA2 on polarization of TAM ...
1. Introduction The uptake of large amounts of lipids from plasma lipoproteins via endocytosis by macrophages in the artery wall to form lipid-engorged foam cells is an important initiating event in the development of atherosclerosis, a major cause of heart disease. The main lipoprotein implicated ...
T cellsBackground SAMHD1 counteracts HIV-1 or HIV-2/SIVsmm that lacks Vpx by depleting the intracellular pool of nucleotides in myeloid cells and CD4+ quiescent T cells, thereby inhibiting the synthesis of retroviral DNA by reverse transcriptase. Depletion of nucleotides has been shown to ...
Full size image PE-EVs induce decreased chemotactic and migratory activity and an increased adhesiveness of THP-1 cells The chemotactic effects of circulating EVs were determined by a Neuro Probe chamber assay. As shown in Fig. 2A, the 12.5 K fraction of HP-EVs exerted a significant chemoat...
The inhibitory rate of the secretion of proMMP-9, the activated MMP-9 and proMMP-2 in the differentiated THP-1 cells was 52.90%, 53.79% and 47.80%, respectively. The inhibitory rate of invasive potential in the undifferentiated cells and the differentiated THP-1 cells was 41.82 % and ...