Monocytes are circulatory precursor cells from myeloid origin that can develop into macrophages or dendritic cells upon migration from the blood stream to tissues. Both macrophages and dendritic cells are professional antigen-presenting cells. Monocytes
Full size image In vivo study of the effects of BCG induced inflammation and the therapeutic potential of targeting DUSP1 Three small interfering RNA sequences were designed and synthesized based on the murine-derived DUSP1 sequence, and siRNA-DUSP1 was transfected into RAW264.7 cells. The knockdo...
When an autophagic stimulus occurs, LC3-I is conjugated to the phosphatidyletha- nolamine (PE) to be converted into form II (LC3-II) which associates with the autophagosomal membrane. First, we infected THP-1 cells with HSV-1 at the multiplicity of infection (MOI) 50, and we evaluated ...
anthracis Δ Sterne, in serum-free media with or without 1 × 10 6 THP-1 cells, and at multiplicities of infection (MOI) of either 1:1 or 1:10 of THP-1 to bacterium. Full size image Principal component analysis indicates a suppressed response to toxigenic strains during early (2 h) ...
In another ELISA to detect the immune-related form of OPN, IC50 values were 0.6, 1.2, and 2.5渭M for compounds C, D, and E, respectively. The decreases in OPN expression and synthesis were confirmed using immunofluorescence and western blot studies using compound-treated cells or cell lysates...
Interferon regulatory factor 7 (IRF7)is a transcription factor involved in the activation of the type I IFN response upon pathogenic infection [1, 2]. IRF7 is constitutively expressed by plasmacytoid dendritic cells (pDCs), which are known as ‘professional’ type I IFN producing cells [1]. ...
The inhibitory rate of the secretion of proMMP-9, the activated MMP-9 and proMMP-2 in the differentiated THP-1 cells was 52.90%, 53.79% and 47.80%, respectively. The inhibitory rate of invasive potential in the undifferentiated cells and the differentiated THP-1 cells was 41.82 % and ...
(Fig.3). Under physiological shear-stress conditions, non-activated monocytic cells did not adhere to ICAM-1 in relevant numbers, either with or without ADP stimulation (THP-1 monocytes/cm2: THP-1 monocytes vs. THP-1 monocytes + ADP: 16.9 + 6.3 vs. 19.9 ± 6.5,p >...
Full size image F.nucleatuminfection induces IDO expression in THP-1-derived macrophages IDO expression in dTHP1 cells treated with live or heat-killedFnwas assessed by qRT-PCR and western blot analysis, respectively. The IDO mRNA and protein assays revealed that IDO can be induced in dTHP1 ce...
D Knockdown of HHLA2 inhibited the activity of AKT/mTOR signaling pathway in AsPC-1 and Capan-2 cells. *p<0.05, **p<0.01, ***p<0.001, ****p<0.0001 Full size image Silencing of HHLA2 in PC cells inhibits M2 polarization of macrophages Finally, effects of HHLA2 on polarization of TAM ...