THP1-Dual™ KO-IRF7 cells were generated from the THP1-Dual™ cell line through the stable knockout of the IRF7 gene. They feature two inducible reporter genes, allowing the concomitant study of the IRF and NF-κB pathways, by monitoring the Lucia luciferase and SEAP (secreted embryonic...
You have full access to this open access chapter,Download chapter PDF Keywords THP-1 U937 Cell line Monocyte Macrophage 14.1Origin and Some Features of THP-1 and U937 Cells The THP-1 cell line is a human monocytic leukaemia cell line which was established in 1980 by Tsuchiya et al. (1980...
Full size image Establishment of a model of THP-1 cell infection by siRNA-DUSP1 combined with BCG Given that BCG infection can upregulate DUSP1 expression, we hypothesized that DUSP1 may be involved in BCG-induced apoptosis and inflammatory responses. We designed and synthesized three small interf...
When an autophagic stimulus occurs, LC3-I is conjugated to the phosphatidyletha- nolamine (PE) to be converted into form II (LC3-II) which associates with the autophagosomal membrane. First, we infected THP-1 cells with HSV-1 at the multiplicity of infection (MOI) 50, and we evaluated ...
Full size image Anti-apoptotic and stress-responses are favored during toxigenic challenge, while cell cycle/differentiation responses are favored during non-toxigenic challenge To further examine specific differences in host response to each strain, an Analysis of Variance (ANOVA) was performed and gene...
et al. Expression of the α7 nAChR subunit duplicate form (CHRFAM7A) is down-regulated in the monocytic cell line THP-1 on treatment with LPS. J. Neuroimmunol. 230, 74-84 (2011).Benfante R, Antonini RA, De Pizzol M, Gotti C, Clementi F, Locati M, Fornasari D (2011) ...
Full size image Effects of siHHLA2 on the migration and invasion of PC cells To clarify the effects of HHLA2 on PC cell metastasis, wound healing and transwell assays have been conducted. As shown in Fig. 6A and B, compared with the NC group, the wound healing rate of siHHLA2-2 grou...
Download: Download full-size image Introduction Visceral infection is the most severe clinical form of leishmaniasis, being caused by L. donovani in the Indian subcontinent. Fortunately, it is curable in most of the cases, but if left untreated, it ultimately leads to death. Globally, about 50...
Within this research we have shown that lumogallion provides an excellent molecular probe for ABA, allowing for the fate of ABA to be deter- mined in a relevant in vitro cell line32. The adsorption of inherent antigen in the form of serum proteins including bovine serum albumin included in...
et al. Expression of the α7 nAChR subunit duplicate form (CHRFAM7A) is down-regulated in the monocytic cell line THP-1 on treatment with LPS. J. Neuroimmunol. 230, 74–84 (2011). Article CAS PubMed Google Scholar Lewis, R. J. Conotoxins as selective inhibitors of neuronal ion ...