THP1-Dual™ hTLR9 cells derive from THP-1 monocytes and overexpress the human TLR9 gene. They also feature two inducible reporter genes, allowing the concomitant study of the IRF and NF-κB pathways, by monitoring the Lucia luciferase and SEAP (secrete
Dual Reporter THP-1 Cells with stable overexpression of TLR7 ± a knockout of TLR8. These cells allow the simultaneous study of NF-kB and IRF pathways.
we first overexpressed or knocked-down miR-574-5p in human monocytic THP1 cells (Fig.3a). Luciferase reporter assays indicated that overexpression of miR-574-5p significantly increased whereas inhibition of miR-574-5p significantly suppressed the transcriptional activity mediated by NFκB and the inte...
THP-1, and U-937 cells have utilized limiting-dilution seeding for the generation of monoclonal cell lines. CRISPR-Cas9-mediated gene editing via non-homologous end joining induces spontaneous insertions or deletions (indels) in the targeted gene. Thus, this approach generally results in a polyclon...
These two shRNAs were chosen for construction of lentiviral vectors for E2-2 knockdown in GEN2.2 cells. The lentiviral vector carries independent GFP expression cassette, which is a reporter and selection marker for cells successfully transduced by E2-2 shRNA. GEN2.2 cells can hardly be ...
Nuclear extracts from LPS-stimulated THP-1 cells were used as positive controls. As an additional control, samples were incubated with an excess (10×, 100×) of nonlabeled κB oligonucleotide, which completely abolished binding of the radiolabeled oligonucleotide to the nuclear proteins. To control...
Ligation of hTLR8 by miR-574-5p induces potent immune or inflammatory responses in human cells To evaluate the functional significance of the binding of miR-574-5p to hTLR8, we first overexpressed or knocked-down miR-574-5p in human monocytic THP1 cells (Fig.3a). Luciferase reporter assays...
(c) NFkB/AP-1 activity of THP1 reporter cells incubated overnight with a range of LPS with or without FYT21 or HVF18. (d) Cells were incubated with 10 mM FYT21 for 1 h, followed by adding LPS directly to the cells (Not Washed) or washing the cells first to remove unbound ...
IRAK-2 transcription appears to be highly cell-specific as 10-fold differences in the same IRAK-2 immediate promoter construct between A549 and THP-1 cells have been noted (45). The current data support previous observations that the IRAK-2-NF-κB signaling loop is strongly self-reinforcing. ...
THP1-Dual™ hTLR3 cells derive from THP-1 monocytes and overexpress the human TLR3 gene as well as all elements important in its signaling cascade. They also feature two inducible reporter genes, allowing the concomitant study of the IRF and NF-κB pat