We looked for a soluble negative regulator that could function to inhibit IL-2 activity in the bioassay, but could find no evidence for one. Even so, the notion that there must be a system to negatively regulate the IL-2-promoted proliferative expansion of antigen-activated T cell clones ...
View LargeDownload Tumor necrosis factor α (TNF-α) bioassay results. A TNF-dependent cell lytic assay was performed with mouse L-929 fibroblasts used as the cell target.31 Cells exposed to culture medium alone are set at 0% lysis, and those exposed to 3-mol/L guanidine hydrochloride provi...
T-Cell Engagers Based Bioassay for Evaluation of PD-1/PD-L1 Inhibitors Activity Article 13 July 2019 Generation of T-cell-redirecting bispecific antibodies with differentiated profiles of cytokine release and biodistribution by CD3 affinity tuning Article Open access 13 July 2021 Bispecific T cel...
Target cell-bound CC-3 was capable of inducing TCR/CD3 engagement in a concentration-dependent manner, as shown by a T cell activation bioassay (Fig. 3A). Next, we used the Jurkat reporter cell line J76TPR [35] to determine CC-3 mediated induction of the transcription factors NFAT (GFP...
Ectopic expression of RORγt protects T cell hybridomas from activation-induced cell death by inhibiting the upregulation of Fas ligand. Following hybridoma stimulation, RORγt also inhibits IL-2 production but does not affect the induction of Nur-77 and Egr-3 nor the upregulation of CD69. ...
cell receptors causing T-cell activation in the absence of target cells. We, therefore, subtracted the background signal generated by the PTEs that carried only T-cell antibodies (Supplementary Fig.6). Here aCD19 × aCD3 constructs lead to the strongest T-cell activation, whereas aCD22 × ...
High-throughput and high-content bioassay enables tuning of polyester nanoparticles for cellular uptake, endosomal escape, and systemic in vivo delivery of mRNA. Sci. Adv. 8, eabk2855 (2022). Article CAS Google Scholar Zha, M. et al. An ester-substituted semiconducting polymer with efficient ...
The ability of 3A9 cells to present the acquired MHC II:peptide complexes to another T cell with the same antigen specificity was determined by their ability to induce IL-2 production by the fresh responder cells using the CTLL-2 bioassay. Figure 4A shows the positive control results of ...
15. A library of cell clones expressing TCRs comprising a population of cell clones expressing 45 different TCR α chains and a population of cell clones expressing 47 different TCR β chains, wherein each of the cell clones expressing different TCR α chains comprises a construct encoding one ...
For T-cell hybridomas, GFP+ cells were isolated by FACS sorting (FACSVantage; BD-Biosciences) after retroviral infection and then stimulated with antigen as previously described.35 Cell supernatants were harvested and assayed for IL-2 content by CTLL-2 bioassay and by ELISA (OPTIEIA; BD-...