原代T细胞或PBMC及Jurkat T 细胞激活测试(T Cell Activation Assay) T细胞或PBMC活化测定试验确定受试品对T细胞或PBMC在细胞活化过程中的调节作用。 东大生物的方法可以评估测试品在由CD3 / CD28抗体诱导下的T细胞或PBMC 活化中的作用,测试方式包括用细胞因子的ELISA或流式细胞仪(FCM)的测定。 案例一:原代T细胞激活后...
CFSE kit来自于thermo, 以下为实验步骤: CFSE assay For new CFSE tube, add 18 ul DMSO into the tube, to get 5 mM (working solution 1:1,000 dilute) 1 mL complete RPMI for 10e6 cell; 0.5 ml for < 5e6 cell 1.1 ul 5mM in 110 ul PBS In tube bottom, put 1ml cells In tube top, ...
ProMab offers T cell activation and proliferation assays to validate the anti-tumor activity of immuno-oncology (IO) drugs. We can provide commercial kits to conduct the assay or can also customize the assay for research and development projects. Request A Quote ProMab Biotechnologies Custom T Cel...
CD3, CD4, CD8 T cell expansion Optimal bead:cell ratio T cell stimulation assay Expansion of human T cells. 视频 文件和下载 证书 搜索 批号Certificate TypeDateCatalog Number(s) 3108747Certificate of Analysis2024年12月17日11131D 3091580Certificate of Analysis2024年11月25日11161D ...
T-细胞激活连接蛋白(LAT)检测试剂盒 Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Linker For Activation Of T-Cell (LAT) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, midd...
HER2-XPAT-NoClvSite induced no detectable T-cell activation, indicating the minimal response with HER2-XPAT protein was likely driven by cleavage resulting from active proteases released during the assay. T-cell activation by unmasked HER2-TCE was negligible in the absence of HER2-expressing BT-...
T-cell activation assay The expression of interleukin 2, as an indicator for T-cell activation, was analysed using T-Cell Activation Bioassays (Promega, J1655)35. The experiment was performed according to the supplier’s instructions. Briefly, CD19-expressing target cells (NALM-6) were added ...
Dynabeads™ 小鼠 T 活化剂 CD3/CD28 用于供研究使用的小鼠 T 细胞的活化与扩增。使用Dynabeads™ 小鼠 T 活化剂 CD3/CD28,您可以:• 无需饲养层细胞即可活化 T 细胞• 活化细胞仍保留体内类似功能• 获得极高可重现性,不会受到可溶性抗体或有丝分裂原污染• 实现从100倍到1000倍的 T 细胞扩增...
T cell activation is predicated upon T cell receptor (TCR) mediated recognition of a cognate peptide presented by the major histocompatibility complex (MHC) of a target antigen presenting cell (APC). From:Progress in Molecular Biology and Translational Science,2019 ...
Simple Plex assays on the Ella platform offer a rapid and precise assessment of secreted T cell activation markers.