Thereby it can be determined whether the dendritic cell have a high capability to activate T-cells and Natural Killer (NK) cells. The invention also encompasses a method for stimulating dendritic cells comprising the step of stimulating the dendritic cells with soluble CD40L and TLR7/8 agonist....
were inoculated with 5 × 105MC38 cells. Viability of DCs was analyzed by flow cytometry on day 14. *p = 0.0322.eDCs purified from conditional knockout mice or B16 cells were treated with IFN-γ for 96 h. Cell viability was determined by MTT assay (n = 3 cells). **p...
Detection of rare antigen-presenting cells by the lacZ T-cell activation assay suggests an expression cloning strategy for T-cell antigens. Proc. Natl Acad. Sci. USA 89, 6020–6024 (1992). Article CAS ADS Google Scholar Gilabert-Oriol, R. et al. Reporter assay for endo/lysosomal escape ...
Anti-IL-1Ra neutralizing antibodies or RPE cells from IL-1Ra knockout donors were used for the assay.Cultured RPE cells greatly suppressed the activation of mDCs, especially the production of pro-inflammatory cytokines, and the expression of cell-surface molecules. Moreover, RPE cells significantly...
The lymphoproliferation assay (a marker of T cell activation triggered by APCs) [65] was not inhibited in both healthy subjects and allergic patients when co-cultured with CAPE treated DCs either (Figure 4B). This result might be explained by the fact that the coculture system was in a "...
Finally, DC depletion abrogated the T cell activation in vivo. Conclusion We present the first in vivo evidence that among hematolymphoid cells, DCs are the most susceptible targets for MVA infection, and DC-mediated Ag presentation is required for the induction of MVA-specific immune responses. ...
BCL2 inhibition reveals a dendritic cell-specific immune checkpoint that controls tumor immunosurveillance. Cancer Discov 2023. Liu P, Zhao L, Zitvogel L, Kepp O, Kroemer G. The BCL2 inhibitor venetoclax mediates anticancer effects through dendritic cell activation. Cell Death Differ 2023. Le Naour...
Patients with non-small cell lung cancer (NSCLC) typically have a significantly poorer prognosis, despite major advances in the detection, treatment, and management of this deadly disease [1]. While immune checkpoint inhibitors such as ipilimumab and nivolumab have recently been shown to prolong ...
F. Inflammatory cytokines as a third signal for T cell activation. Curr. Opin. Immunol. 22, 333–340 (2010). Article CAS PubMed PubMed Central Google Scholar León, B., López-Bravo, M. & Ardavín, C. Monocyte-derived dendritic cells formed at the infection site control the induction...
(Fig. 2d), and they effectively downregulated B cell FcγRII and induced B-cell activation (Fig. 2eandSupplementary Fig. 2c,d). In the culture system of TDCs and B cells, we did not detect a marked apoptosis of B cells, as assessed by determining the number of dead trypan blue ...