CFSE assay For new CFSE tube, add 18 ul DMSO into the tube, to get 5 mM (working solution 1:1,000 dilute) 1 mL complete RPMI for 10e6 cell; 0.5 ml for < 5e6 cell 1.1 ul 5mM in 110 ul PBS In tube bottom, put 1ml cells In tube top, put 110 ul PBS Quickly mix 20min ...
T cell activation with Dynabeads magnetic beads Dynabeads T cell activation and expansion products mimicin vivoT cell activation from antigen-presenting cells (APCs). Key benefits of Dynabeads T cell activation and expansion kits include: Easy protocol—No need to prepare autologous APCs,...
英文名称: ELISA Kit for Regulated On Activation In Normal T-Cell Expressed And Secreted (RANTES) 分子式: 电询 纯度: 99 % 产地: 中国/美国/德国 品牌: 上海信裕 包装规格: 96T/48T 货号: XYEA116Hu 别名: 电询 调节激活正常T-细胞表达分泌因子(RANTES)检测试剂盒 适用生物 Homo sapiens ...
Here we provide a detailed protocol for APC-ms synthesis and use for human T-cell activation, and discuss important considerations for material design and T-cell co-culture. This protocol describes the facile assembly of APC-ms in ~4 h and rapid expansion or enrichment of relevant T-cell ...
赛默飞的CTS; OpTmizer; T 细胞扩增 SFM 培养基,瓶装,是专为人类T淋巴细胞的生长和扩增而开发的。OpTmizer CTS T-Cell Expansion Medium是一种完整的无血清,无异种1X培养基,包含OpTmizer T-Ce。欢迎咨询和购买。
T cell stimulation in vitro Splenic CD8+T cells were isolated with EasySep Mouse CD8+T cell Isolation Kit and stimulated with 5 μg/ml anti-CD3 (coated on plate) and 2 μg/ml anti-CD28 (soluble) according to standard protocol. Forty-eight hours after stimulation, T cells were harves...
英文全称:Inducible T-Cell Co Stimulator Ligand 检测范围:0.25ng/ml~8ng/ml 灵敏度:0.1 货号: FS15675 试剂准备: 1. 使用前将所有的试剂和标本缓慢均衡至室温(18-25oC),试剂不能直接在37oC溶解。 2. 标准品(冻干品):每瓶标准品加入标准品稀释液1mL,盖好后室温静置大约10分钟,同时反复颠倒/搓动以助溶解...
During T-cell activation, CD69 and CD25 are expressed on the T-cell surface [26]. Therefore, we speculated that CAR- and CR-co-expressing T cells synergistically contribute to the activation of T cells. After the co-culture of engineered T cells with tumor cells for 24 h, we measured ...
B7-mediated signaling is required for T cell immunity, we then investigated the biological effects of M2 on hB7.1/2 costimulatory functions via an ex vivo T cell activation assay. Purified T cells from peripheral blood mononuclear cells (PBMC) of healthy humans were co-incubated with hB7.1/2-...
T cell activation is a cornerstone in manufacturing of T cell-based therapies, and precise control over T cell activation is important in the development of the next generation T-cell based therapeutics. This need cannot be fulfilled by currently available methods for T cell stimulation, in partic...