原代T细胞或PBMC及Jurkat T 细胞激活测试(T Cell Activation Assay) T细胞或PBMC活化测定试验确定受试品对T细胞或PBMC在细胞活化过程中的调节作用。 东大生物的方法可以评估测试品在由CD3 / CD28抗体诱导下的T细胞或PBMC 活化中的作用,测试方式包括用细胞因子的ELISA或流式细胞仪(FCM)的测定。 案例一:原代T细胞激活后...
CFSE kit来自于thermo, 以下为实验步骤: CFSE assay For new CFSE tube, add 18 ul DMSO into the tube, to get 5 mM (working solution 1:1,000 dilute) 1 mL complete RPMI for 10e6 cell; 0.5 ml for < 5e6 cell 1.1 ul 5mM in 110 ul PBS In tube bottom, put 1ml cells In tube top, ...
英文名称: ELISA Kit for Linker For Activation Of T-Cell (LAT) 分子式: 电询 纯度: 99 % 产地: 中国/美国/德国 品牌: 上海信裕 包装规格: 96T/48T 货号: XYEA270Hu 别名: 电询 T-细胞激活连接蛋白(LAT)检测试剂盒 适用生物 Homo sapiens (Human,人) T-细胞激活连接蛋白(LAT)检测试剂...
ProMab offers T cell activation and proliferation assays to validate the anti-tumor activity of immuno-oncology (IO) drugs. We can provide commercial kits to conduct the assay or can also customize the assay for research and development projects. Request A Quote ProMab Biotechnologies Custom T Cel...
Here we provide a detailed protocol for APC-ms synthesis and use for human T-cell activation, and discuss important considerations for material design and T-cell co-culture. This protocol describes the facile assembly of APC-ms in ~4 h and rapid expansion or enrichment of relevant T-cell ...
T cell activation with Dynabeads magnetic beads Dynabeads T cell activation and expansion products mimicin vivoT cell activation from antigen-presenting cells (APCs). Key benefits of Dynabeads T cell activation and expansion kits include: Easy protocol—No need to prepare autologous APCs,...
英文名称:Monoclonal Antibody to Linker For Activation Of T-Cell (LAT) 分子式:电询 纯度:100 % 产地:进口/国产 品牌:钰博生物 包装规格:0.1ml/0.2ml 别名:电询 T-细胞激活连接蛋白(LAT)单克隆抗体 FOR IN VITRO AND RESEARCH USE ONLY, NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES!
T cell stimulation in vitro Splenic CD8+T cells were isolated with EasySep Mouse CD8+T cell Isolation Kit and stimulated with 5 μg/ml anti-CD3 (coated on plate) and 2 μg/ml anti-CD28 (soluble) according to standard protocol. Forty-eight hours after stimulation, T cells were harves...
T 细胞介导的杀伤试验是其中之一InnoModels Biotechnology 的研究项目。该检测利用T细胞作为杀死肿瘤细胞的工具,具有很高的功效和潜力。通过这项检测,InnoModels Biotechnology 旨在开发更有效的抗癌疗法。 T 细胞介导的杀伤实验的第一步是收集患者自身的 T 细胞。然后这些T细胞经过一系列处理和培养以增强其杀伤能力。接下来...
Granzyme B has been described as CGL1 (cathepsin G-like-1), a serine protease expressed only in cytotoxic T-lymphocytes after cell activation, and CTLA-1 (cytotoxic T lymphocyte-associated serine esterase 1) based on identification of mRNA in various cytotoxic...