Target cell-bound CC-3 was capable of inducing TCR/CD3 engagement in a concentration-dependent manner, as shown by a T cell activation bioassay (Fig. 3A). Next, we used the Jurkat reporter cell line J76TPR [35] to determine CC-3 mediated induction of the transcription factors NFAT (GFP...
cell receptors causing T-cell activation in the absence of target cells. We, therefore, subtracted the background signal generated by the PTEs that carried only T-cell antibodies (Supplementary Fig.6). Here aCD19 × aCD3 constructs lead to the strongest T-cell activation, whereas aCD22 × ...
T-Cell Engagers Based Bioassay for Evaluation of PD-1/PD-L1 Inhibitors Activity Article 13 July 2019 Generation of T-cell-redirecting bispecific antibodies with differentiated profiles of cytokine release and biodistribution by CD3 affinity tuning Article Open access 13 July 2021 Bispecific T cel...
Development of a bioassay to detect T-cell-activating impurities for T-cell-dependent bispecific antibodies 来自 Springer 喜欢 0 阅读量: 52 作者:HY Lee,E Contreras,AC Register,Q Wu,K Abadie,K Garcia,PY Wong,G Jiang 摘要: T-cell-dependent bispecific antibodies (TDBs) are promising cancer ...
and an antigen in a form suitable to trigger a primary activation signal in the T cell when complexed with the TCR/CD3 complex, wherein said agent is attached on a surface; and (b) stimulating a CD28 accessory molecule on the surface of the T cells in vitro with a ligand that binds ...
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Doecke, et al., The identifi- cation of a small molecule inhibitor that specifically reduces T cell-mediated adap- tive but not LPS-mediated innate immunity by T cell membrane-monocyte contact bioassay, Immunol. Lett. 117 (2008) 114-118....
View LargeDownload Tumor necrosis factor α (TNF-α) bioassay results. A TNF-dependent cell lytic assay was performed with mouse L-929 fibroblasts used as the cell target.31 Cells exposed to culture medium alone are set at 0% lysis, and those exposed to 3-mol/L guanidine hydrochloride provi...
The biological activity determined by a chemotaxis bioassay using human T-lymphocytes is in a concentration range of 1.0-10ng/ml.Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder.Formulation Lyophilized from a 0.2μm filtered concentrated solution in 20mM PB, pH7.4, 150mM ...
Standard material was served as a bioassay calibrant with 40,000 IU for measuring the potency of rhIFN. In this assay, IU was calculated as follows: Sample activity (IU/ml) =Standard activity (IU/ml) of 1 strow X 2 (Nsam-Nstd)X Dilution fold number of 1 strow sample (Ac: ...