Reporter cell assays To determine the capacity of CC-3 to induce T cell responses, a T cell activation bioassay (Promega, Madison, WI) was used according to manufacturer’s instructions. Briefly, 50,000 BC cells per well were seeded the day before. Then CC-3 in a concentration range of ...
T-cell activation assay The expression of interleukin 2, as an indicator for T-cell activation, was analysed using T-Cell Activation Bioassays (Promega, J1655)35. The experiment was performed according to the supplier’s instructions. Briefly, CD19-expressing target cells (NALM-6) were added ...
Figure 1. TNF production by T-cell clones. HuT 78 and K-4 cells were incubated for 48 h in medium and TNF levels in supernatants measured by L929 bioassay (A) or ELISA (B). Mean ± S.E. of three experiments. IL2 was not detected in supernatants of resting HuT 78, K-4, or Jurk...
In addition, we were unable to detect sufficient levels of engager molecules in the peripheral blood of mice using a bioassay in which human T cells are mixed with EphA2-positive tumor cells and serum of mice (Supplementary Figure S10). While these results are encouraging in regards to the ...
The PD-1/PD-L1 blockade bioassay from Promega (thaw-and-use format) was performed according to the manufacturer’s instructions. The assay is based on a two-cell system requiring CHO cells expressing both PD-L1 and a T cell activator, and a Jurkat/NFAT-RE Reporter Cell Line overexpressing...
A new bioassay for human granulocyte colony-stimulating factor (hG-CSF) using murine myeloblastic NFS −60 cells as targets and estimation of its levels in sera from normal healthy persons and patients with infectious and hematological disorders. Exp Hematol. 1989;17(2):116–9. PubMed CAS ...
TGF-β production by the tumor cell lines was quantified using a highly sensitive and specific, nonradioactive, bioassay[27]. This bioassay is based on the ability of TGF-β to induce PAI-1 expression. Briefly, MLECs stably transfected with a construct containing the human PAI-1 promoter fus...
TGF-β production by the tumor cell lines was quantified using a highly sensitive and specific, nonradioactive, bioassay[27]. This bioassay is based on the ability of TGF-β to induce PAI-1 expression. Briefly, MLECs stably transfected with a construct containing the human PAI-1 promoter fus...
A Vesicular Stomatitis Virus Replicon-Based Bioassay for the Rapid and Sensitive Determination of Multi-Species Type I Interferon. Plos One, 6 (2011), Article e25858 CrossrefGoogle Scholar 50 S.F. Ahmed, A.A. Quadeer, M.R. McKay Preliminary Identification of Potential Vaccine Targets for the...
T cell subsets and activated T cells were identified by flow cytometry (BD FACSCanto II) with the aid of FlowJo 10 software (FlowJo, LLC). 2.13. PD-1/PD-L1 blockade bioassay PD-1/PD-L1 blockade bioassays was performed according to the manufacturer's protocol (#J1250, Promega) [17]....