Toxicity of E-liquid Flavors and Base Components on the AGS and A549 Cell Lines At the 4 hour and 24 hour time points the flavorings were shown to be the most harmful part of the e-liquid on AGS and A549 cells. The second assay was a CytoTox 96 Non-Radioactive Cytotoxicity Assay to...
The assay provides a luminogenic caspase-3/7 substrate, which contains the tetrapeptide sequence DEVD, in a reagent optimized for caspase activity, luciferase activity and ce 6、ll lysis. Adding a singleCaspase-Glo ? 3/7 Reagent in an “add-mix-measure” format results in cell lysis, ...
Five different commonly used mammalian cell lines were transfected with the plasmid, and transfection efficiency was assessed by measuring the luciferase activity using the ONE-Glo™ Luciferase Assay System (Cat.# E6110; n = 6). The PureYield™ Plasmid Midiprep System is designed for ...
Continue reading“About the Development of an Improved BRET Assay: NanoBRET” Robert Hooke first coined the term “cell” after observing plant cell walls through a light microscope—little empty chambers, fixed in time and space. However, cells are anything but fixed. Cells are dynamic: continual...
Cell Death and Differentiation 24, 546–58. Are you looking for proteases to use in your research? Explore our portfolio of proteases today. Share this: Facebook LinkedIn Twitter WhatsApp Reddit Email Print Like this: Analysis of a biosimilar mAb using Mass Spectrometry Posted on October 4,...
Integrated stress response plasticity governs normal cell adaptation to chronic stress via the PP2A-TFE3-ATF4 pathway. View PDF InCell Death and Differentiationon 1 December 2024 by Avelar, R. A., Gupta, R., et al. Proteomics analysis reveals the differential impact of the p97 inhibitor CB-50...
(GSH) form. A change in GSH and GSSG levels are important when assessing toxicological responses and an indicator of oxidative stress, potentially leading to apoptosis or cell death. The GSH and GSSG detection assay provides a simple, rapid multiwell-plate format where stable luminescent signals ...
Adria Dangla-Valls, Philip Hublitz, Menelaos Pangalos, Simon Lovestone, Elena M. Ribe, Noel J. BuckleyBSA). Cell deathassayCell death was quantified using theCytoTox-Glo Cytotoxicity Assay(Promega,G9291) according to manufacturer's instructions. For all treatment conditions, cell death was compa...
Revised9/15 TM058 TECHNICALMANUAL Dual-Glo ® Luciferase AssaySystem InstructionsforuseofProducts E2920,E2940andE2980. PromegaCorporaton·2800WoodsHollowRoad·Madison,WI53711-5399USA·TollFreeinUSA800-356-9526·608-274-4330·Fax608-277-25161 .promegaTM058·Revised9/15 1.Description...2 2.Produc...
Paucek P, Beseler C, Boisen M, Bubak A, Asturias F, George W, Graner A, Ormond D,et al.respectively. Apoptosis/NecrosisassaysTheRealTime-Glo Annexin V Apoptosis and Necrosis Assaykit (Promega#JA1011) was used for evaluating apoptotic and necrotic cell death. The five components ...