t-cell-activation-in-vitro
If a retrovirus vector is to be used for this transduction, activation of the T-cells is required for integration of the transgene to occur. The activation protocol should ensure expansion of a broad repertoire of donor T-cells. Notably, T-cells specific for herpes virus family antigens are ...
In vitrocell differentiation and cell separation assays are easier to perform and offer greater control in experimentation compared to theirin vivocounterparts, makingin vitroassays especially useful in the early, pre-clinical phases of therapeutics research. Leveraging innovative microbubble...
In conclusion, T-cells were activated by swine dustin vitro . Thus, our previous findings of T-cell activation following swine dust exposure, in vivo may be an effect of the dust either directly on T-cells or on other cells which in turn contribute to the T-cell activation....
Thus, activation of professional APCs was mandatory for induction of effector CD8 + T cell responses in vivo, while this step was largely dispensable in vitro. 展开 关键词: APC CpG VLP in vivo T cell activation in vitro T cell activation ...
64Cu-intraperitoneal radioimmunotherapy;in vitro tumor cell-binding assay;pancreatic cancer;peritoneal dissemination 1. Introduction Pancreatic cancer has a dismal prognosis, with an overall 5-year survival rate below 10% [1,2,3,4]. Surgical resection following chemotherapy with gemcitabine is the prima...
It is expressed in T cells and binds to its ligand PD-L1, which is mainly expressed in tumor cells, thus preventing T-cell activation and inducing immunological exhaustion [19,20,21] (Figure 1). Figure 1. Scheme of co-stimulatory and co-inhibitory receptors implicated in the immune ...
免疫原:纯化的人T淋巴细胞 报告应用:体内T细胞耗竭 体内预防移植排斥反应 体外抑制混合淋巴细胞反应(MLR) 功能实验 免疫组化(冰冻切片) 酶联免疫吸附试验(ELISA) 配方:PBS,pH 7.0 不含稳定剂或防腐剂 内毒素:<2EU/mg (<0.002EU/μg) 由LAL凝胶凝集试验确定 ...
Recent advances in theranostic nanomedicine can promote stem cell and immune cell-based therapy. Gold nanoparticles (GNPs) have been shown to be promising agents for in-vivo cell-tracking in cell-based therapy applications. Yet a crucial challenge is to develop a reliable protocol for cell upload...