原代T细胞或PBMC及Jurkat T 细胞激活测试(T Cell Activation Assay) T细胞或PBMC活化测定试验确定受试品对T细胞或PBMC在细胞活化过程中的调节作用。 东大生物的方法可以评估测试品在由CD3 / CD28抗体诱导下的T细胞或PBMC 活化中的作用,测试方式包括用细胞因子的ELISA或流式细胞仪(FCM)的测定。 案例一:原代T细胞激活后...
CFSE kit来自于thermo, 以下为实验步骤: CFSE assay For new CFSE tube, add 18 ul DMSO into the tube, to get 5 mM (working solution 1:1,000 dilute) 1 mL complete RPMI for 10e6 cell; 0.5 ml for < 5e6 cell 1.1 ul 5mM in 110 ul PBS In tube bottom, put 1ml cells In tube top, ...
T cell function and fate can be influenced by several metabolites: in some cases, acting through enzymatic inhibition of α-ketoglutarate-dependent dioxygenases, in others, through post-translational modification of lysines in important targets. We show here that glutarate, a product of amino acid ...
T-cell activation, and cytotoxic function markers, suggesting an important role of these factors in modulating a coordinated immune-mediated anti-tumor T-cell response. The strong positive correlation between TILs and 4-1BB/OX-40 expression (Fig.1e) likely explains the association with 4-1BB/OX...
T-细胞激活连接蛋白(LAT)检测试剂盒 Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Linker For Activation Of T-Cell (LAT) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, midd...
To confirm T-cell changes previously reported in atopic eczema, to compare T-cell subset values in atopic and seborrheic infants, and to determine whether T-cell subset assay would be a useful differentiating marker between atopic and seborrheic infants, three age- and sex-matched groups of norma...
[1] Mohammed, Somala, et al. "Improving chimeric antigen receptor-modified T cell function by reversing the immunosuppressive tumor microenvironment of pancreatic cancer." Molecular Therapy 25.1 (2017): 249-258. [2] Jackson, Hollie J., Sarwish Rafiq, and Renier J. Brentjens. "Driving CAR T...
Critically, RelB within APCs was required for the expansion of donor helper T cell type 1 (Th1) effectors and subsequent alloreactivity, but not the peripheral expansion or function of donor FoxP3+ regulatory T cells. These data suggest that the targeted inhibition of nuclear RelB translocation...
英文全称:Inducible T-Cell Co Stimulator Ligand 检测范围:0.25ng/ml~8ng/ml 灵敏度:0.1 货号: FS15675 试剂准备: 1. 使用前将所有的试剂和标本缓慢均衡至室温(18-25oC),试剂不能直接在37oC溶解。 2. 标准品(冻干品):每瓶标准品加入标准品稀释液1mL,盖好后室温静置大约10分钟,同时反复颠倒/搓动以助溶解...
Shortened intervals during heterologous boosting preserve memory CD8 T cell function but compromise longevity. J. Immunol. 196, 3054–3063 (2016). Article CAS PubMed Google Scholar Rubin, H. Telomerase and cellular lifespan: ending the debate? Nat. Biotechnol. 16, 396–397 (1998). Article ...