Synthetic long-read sequencing reveals intraspecies diversity in the human microbiome. Nature Biotechnology. 2016;34(1):64-69.V. Kuleshov et al. Synthetic long-read sequencing reveals intraspecies diversity in the human microbiome. Nature Biotech, 34(1):64-69, 2016....
专利名称：SYNTHETIC LONG READ DNA SEQUENCING 发明人：EDWARDS, Jeremy Scott 申请号：EP14852570.2 申请日：20140924 公开号：EP3055431A4 公开日：20170510 专利内容由知识产权出版社提供 摘要：The disclosure describes a method for sequencing long portions of DNA sequence by assembling a plurality of shorter...
Long-read sequencing technologies were launched a few years ago, and in contrast with short-read sequencing technologies, they offered a promise of solving... MA Madoui,S Engelen,C Cruaud,... - 《Bmc Genomics》 被引量: 115发表: 2015年 Long read nanopore sequencing for detection of HLA an...
Such an approach can be conducted using both short-read and long-read sequencing data, as well as a combination of both. To asses... Raphael Eisenhofer,Joseph Nesme,Luisa Santos-Bay,... - 《Microbiology Spectrum》 被引量: 0发表: 2024年...
Characterizing the microbial communities inhabiting specimens is one of the primary objectives of microbiome studies. A short-read sequencing platform for reading partial regions of the 16S rRNA gene is most commonly used by reducing the cost burden of n
and immune receptors. These controls provide a known ground-truth reference against which to measure the performance of diverse sequencing technologies, reagents, and bioinformatic tools. Using this approach, we provide a comprehensive evaluation of short- and long-read sequencing instruments, library pre...
We show how the modular rearrangement of synthetic chromosomes by SCRaMbLE can be easily determined using long-read nanopore sequencing and we explore experimental conditions that optimise diversification and screening. This synthetic genome approach to metabolic engineering provides productivity improvements in...
Synthetic oligodeoxyribonucleotides were used as primers for sequencing DNA by the dideoxy chain termination method. We report that the use of unpurified preparations of such oligonucleotides decreases the time involved in preparing the ... R Sanchez-Pescador,MS Urdea - Dna 被引量: 135发表: 1984...
Since the majority of sequence reads from cfDNA are about 167 bp long, only merged reads (where read 1 and read 2 overlap by at least 30 bp of complementarity) were used for downstream analyses. Additional files 1,2 contain the sequencing metrics for all sequenced libraries. The data ...
For example, the latter could be used to19document when the DNA sequencing results for an assembled construct do not match the original target sequence.206.7 ExperimentalData21The purpose of theExperimentalDataclass is to aggregate links to experimental data files. AnExperimentalData22is typically ...